Difference between revisions of "Team:DTU-Denmark/Results-choosing-organism"

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<p>Here you can describe the results of your project and your future plans. </p>
 
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<li> Clearly and objectively describe the results of your work.</li>
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<h3>Describe what your results mean </h3>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<h2 class="media-heading" style="text-align: left;margin-bottom: 35px; color:#50C8E8;">Choice of organism</h2>
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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Growing conditions between fungal species can vary, depending on how they grow in nature. Factors could be temperature, amount of light and the substrate, among other things. With the goal of growing fungal biobricks, a selection of the species used for our research needed to be done.  
  
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<li>A list of linked bullet points of the successful results during your project</li>
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<i>Pleurotus ostreatus</i>, <i>Aspergillus oryzae</i> and <i>Schizophyllum commune</i> were initially investigated. First, <i>S. commune</i> has been investigated and used for the generation of bricks by the company Ecovative Design (1), which we collaborated with. In addition, <i>S. commune</i> also allowed us to study genes affecting the structure of mycelium, by using mutants as ΔSC3. This strain is characterized by a lack of SC3 gene, responsible for the production of hydrophobins. Hydrophobins are small proteins present in filamentous fungi that have the ability to repel water and create a coating in hydrophobic:hydrophilic surfaces (2).
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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Secondly, <i>P. ostreatus</i> is a common edible mushroom also known as oyster mushroom that has been widely cultivated. In addition to having a strong mycelium, it is used in industrial processes and mycoremediation. (3).
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Eventually, <i>A. oryzae</i> has been used for fermentation processes for at least 2000 years (4). In addition, its genome has been sequenced (5), being an optimal choice for the generation of a genetic toolbox.
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<h2 class="media-heading"  style="text-align: right;margin-bottom: 35px; color:#F8A05B;">Results</h2>
  
  
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In the first experiment, the species were seeded at 3 different concentrations of MEA (47,5 g, 50 g and 52,5), PDA (19,5 g, 39 g and 58,5 g) and YPD. Thereafter, growth pictures were taken every 24 hours and the size of the fungal colonies was compared. In addition, different growth conditions (temperature and light) were tested for each of the species. <i>S. commune</i> was incubated 30ºC at light and 27ºC at light, <i>P. ostreatus</i> at 28ºC at dark and 25ºC at light, and <i>A. oryzae</i> at 28ºC at dark and 30ºC at dark.
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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In addition, we also investigated the growth of a mutated <i>S. commune</i> strain ,ΔSC3.
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/e/e7/T--DTU-Denmark--ResultsSpecies1.png" style="max-width: 100%;" > <figcaption><p style="text-align:center; font-size:14px;"><b>Fig. 1: </b> - Growth of <i>A. oryzae</i> on PDA 58.5g (A), <i>S. commune</i> on PDA 39g (B) and  <i>P. ostreatus</i> on PDA 19.5g (C) plates after 24, 48 and 72 hours respectively after inoculation.</p></figcaption>
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<h2 class="media-heading" style="text-align: left;margin-bottom: 35px; color:#50C8E8;">Brick construction</h2>
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To test whether the integration, and possible expression of <i>amilCP</i>, would lead to a visual change in materials produced, a small brick was inoculated using spores from the identified transformants. However, this did not produce any visual obvious change in brick color, see figure 4:
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Figure 4: Tag et billede af murstenen af svamp
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Revision as of 15:58, 16 October 2018

Choice of organism

Choice of organism

Growing conditions between fungal species can vary, depending on how they grow in nature. Factors could be temperature, amount of light and the substrate, among other things. With the goal of growing fungal biobricks, a selection of the species used for our research needed to be done.

Pleurotus ostreatus, Aspergillus oryzae and Schizophyllum commune were initially investigated. First, S. commune has been investigated and used for the generation of bricks by the company Ecovative Design (1), which we collaborated with. In addition, S. commune also allowed us to study genes affecting the structure of mycelium, by using mutants as ΔSC3. This strain is characterized by a lack of SC3 gene, responsible for the production of hydrophobins. Hydrophobins are small proteins present in filamentous fungi that have the ability to repel water and create a coating in hydrophobic:hydrophilic surfaces (2).

Secondly, P. ostreatus is a common edible mushroom also known as oyster mushroom that has been widely cultivated. In addition to having a strong mycelium, it is used in industrial processes and mycoremediation. (3).

Eventually, A. oryzae has been used for fermentation processes for at least 2000 years (4). In addition, its genome has been sequenced (5), being an optimal choice for the generation of a genetic toolbox.

Results

In the first experiment, the species were seeded at 3 different concentrations of MEA (47,5 g, 50 g and 52,5), PDA (19,5 g, 39 g and 58,5 g) and YPD. Thereafter, growth pictures were taken every 24 hours and the size of the fungal colonies was compared. In addition, different growth conditions (temperature and light) were tested for each of the species. S. commune was incubated 30ºC at light and 27ºC at light, P. ostreatus at 28ºC at dark and 25ºC at light, and A. oryzae at 28ºC at dark and 30ºC at dark.

In addition, we also investigated the growth of a mutated S. commune strain ,ΔSC3.

Fig. 1: - Growth of A. oryzae on PDA 58.5g (A), S. commune on PDA 39g (B) and P. ostreatus on PDA 19.5g (C) plates after 24, 48 and 72 hours respectively after inoculation.



Brick construction

To test whether the integration, and possible expression of amilCP, would lead to a visual change in materials produced, a small brick was inoculated using spores from the identified transformants. However, this did not produce any visual obvious change in brick color, see figure 4:

Figure 4: Tag et billede af murstenen af svamp