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<h2>Imperial College London iGEM - Wet Lab</h2> | <h2>Imperial College London iGEM - Wet Lab</h2> | ||
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− | <p> | + | <p>In conjunction with Imperial College, we tested a range of SoxS promoter strengths in order to create a functional library. GFP production was measured in response to hydrogen peroxide (and electrical stimuli on their part) to characterise a range of BLAST SoxS promoters. Our parts were to be assembled by the digestion of eCFP from the kit plate and the amplified promoter construct was digested using XbaI and EcoRI followed by ligation. A kit plate assay was performed to measure GFP expression as a function of both hydrogen peroxide and cell growth in order to determine the cytotoxic effects of the inducer. |
+ | </p> | ||
</ul> | </ul> | ||
</div> | </div> |
Revision as of 19:49, 16 October 2018
Collaborations
UK iGEM Meetup
On 12th and 13th July, we helped host the UK-wide iGEM meetup at Worcester College, Oxford in partnership with SynBio UK. Teams travelled from across the country to attend this two-day event that promoted collaboration, offered advice and gave an insight into what lies beyond the iGEM competition.
Imperial College London iGEM - Wet Lab
In conjunction with Imperial College, we tested a range of SoxS promoter strengths in order to create a functional library. GFP production was measured in response to hydrogen peroxide (and electrical stimuli on their part) to characterise a range of BLAST SoxS promoters. Our parts were to be assembled by the digestion of eCFP from the kit plate and the amplified promoter construct was digested using XbaI and EcoRI followed by ligation. A kit plate assay was performed to measure GFP expression as a function of both hydrogen peroxide and cell growth in order to determine the cytotoxic effects of the inducer.
Imperial College London iGEM - Outreach
New Scientist Live
Imperial College London iGEM - Software Testing
App
METU HS iGEM - Mentoring
High School Mentorship
Tec Monterrey GDL iGEM - ?
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Tec Monterrey iGEM - ?
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Warwick iGEM - Modelling
Hill Functions