Difference between revisions of "Team:UMaryland/BCmodel"
15kuffnerc (Talk | contribs) |
15kuffnerc (Talk | contribs) |
||
| Line 84: | Line 84: | ||
<div class="imageBox"> | <div class="imageBox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/4/40/T--UMaryland--consts.png" style="max-width: 100%" alt="Waluigi Time!"> |
<div class="imageBoxDescription">Equations from the assumption of zero order reaction</div> | <div class="imageBoxDescription">Equations from the assumption of zero order reaction</div> | ||
</div> | </div> | ||
Revision as of 02:33, 17 October 2018
Bacterial Cellulose
Does the addition of a cellulose binding domain accelerate the degradation of PET?
Model Setup
So, with K = 0.00463 s^-1, [PETase] = 6.5uM, and [product] = 0.019M, degradation based on a zero order reaction is expected to take roughly two days.
We considered the following degradation setup: a sheet of PET is “sandwiched” between two layers of bacterial cellulose, to which we add our PETase-CBD which is able to bind to the bacterial cellulose. The bacterial cellulose, due to its insulating nature, will prevent PETase that is close to the PET film to escape into the rest of the solution, resulting in a high local concentration of PETase close to the surface.
Bacterial cellulose creates a "sandwich" to maximize PETase contact with the surface
We determined the surface area of a PET bottle modeling it as a perfect cylinder with diameter d = 7 cm, height h = 23 cm, and mass m = 4 g. which is about the average size of a disposable water bottle.
Surface area of bottle = 2dh = (2)(7π cm)(23 cm) = 322π cm²
Volume of PET = wdh = (0.1 cm)(7π cm)(23 cm) = 16.1π cm3
We want to model the exact difference between the local and the global concentrations in such a scenario. In order to do this, we used an assumed value for the “working thickness” of cellulose, which is the thickness at which making the cellulose layer thicker will yield a small increase in the insulating behavior of the cellulose layers. We used a value that is an underestimate of 0.1 mm as our starting value. We aim to use experimental data to adjust this value. We determined the following parameters:
Cellulose density = 1.5 g/cm3
Total working weight of cellulose = (1.5 g/cm3)(322π cm²)(0.01 cm) = 15.87 g cellulose
Using values obtained from the literature about the CipA-CBD [1] where our CBD was acquired from, we determined:
Maximum binding of CipA-CBD to cellulose = 0.43 µM/g cellulose
Local concentration of PETase at 50% binding = (0.5)(15.87 g)(0.43 µM/g) = 3.41 µM PETase
Enzyme Kinetics
PET is a long polymer, but we will assume complete degradation to MHET (MW = 210.185 g/mol). For a 4g bottle in a 1L container, that comes out to 0.019M
The degradation of PET follows heterogeneous catalysis, where the reactants and the products [2] are in different phases. This creates difficulty in modeling the rate of degradation but teams such as Tianjin China has used Langmuir's Equation, secretion, and diffusion models to model the process of secretion, diffusion, adsorption, and degradation. However, while their models gave great insight into the mathematical relationships between these variables, it was not possible to obtain practical results as many of the variables used in the equation are not found in the literature and are difficult to obtain experimentally.
We have developed the “working cellulose” model which is more practical in predicting the rate of degradation when PETase with a cellulose binding domain is added to a degradation system. We modeled the rate of degradation of PETase as a zero-order system, where the concentration of the reactant is negligible to the rate of the reaction. This is present in many surface-limited chemical catalysis models, where the catalyst surface is saturated by the reactants, and the rate limiting step can be simplified in the surface area of the catalyst.
However, in our PET degradation model, the reverse holds true, where the concentration of PETase is the rate limiting variable compared to the surface area of the PET film when considering practical examples such as the degradation of a plastic bottle. We determined the following rate equation for the degradation of PET:
Equations from the assumption of zero order reaction
In our reduced and simplified zero order enzyme kinetics equation, we only need to know the product, time, and enzyme concentrations to determine the kinetic variable k to predict degradation. Through values obtained through the original PETase discovery paper by Yoshida et al, we were able to calculate the kinetic variable for both low-crystalline and high-crystalline PET.
At 30°C, pH 7.0, [PETase concentration] = 50nM, time = 18 hrs: PETase produced a total of 0.015 mM of product when fed high-crystalline PET and 0.3 mM of product when fed thin-sheet PET.
Using above data we can determine the rate constants.
Equations from the assumption of zero order reaction
We can determine the rate constant of PETase degrading PET with values from Yoshida's 2016 paper. PET was incubated with 50nM PETase at pH 7.0 for 18 hours at 30C. 0.015mM of degradation product was observed from high-crystalline PET, the type found in a bottle. Plugging these parameters into the equation above, we obtained K = 0.00463 s^-1 for high crystalline PET exposed to PEtase.
Contact Us
umarylandigem@gmail.com
Biology - Psychology Building
4094 Campus Dr, College Park, MD 20742
umarylandigem@gmail.com
Biology - Psychology Building
4094 Campus Dr, College Park, MD 20742
© University of Maryland 2018
Follow Us
