Difference between revisions of "Team:Chalmers-Gothenburg/Description"

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             Our engineered yeast cells adhere to the cancer cell surface, this is done by fusing HlpA (Histone Like Protein A) to the anchoring protein Aga2, which attaches to AGA1 on the yeast cell surface. HlpA binds to the protein Syndecan1 on the cancer cell surface.
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             Our engineered yeast adheres to the membrane protein Syndecan1 on the cancer cell surface. To do so, a protein complex is introduced consisting of Histone Like Protein A (HlpA), which specifically binds to Syndecan1, a linker, and the yeast cell surface display protein Aga2.
 
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             For detection, <i>S. cerevisiae</i> produces ultrasound-detectable gas vesicles, a mixed gene set from the gas vesicle operons from <i>Bacillus megaterium</i> and <i>Anabaena flos-aquae</i>. To allow single transcript expression in a eukaryote, 2A viral peptide sequences that cleave themselves are introduced between the genes.
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             <i>S. boulardii</i> produces ultrasound-detectable gas vesicles, a mixed gene-set from prokaryotic gas vesicle operons. To allow single transcript expression in a eukaryote, 2A viral peptide sequences that cleave themselves are introduced between the genes.
 
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             A cancer-targeting protein is introduced into <i>S. cerevisiae</i>. Two options are: myrosinase, an enzyme that converts glucosinolate (present in cruciferous vegetables) into the chemopreventive compound sulphoraphane, or p28, which is a cell-penetrating peptide. The expression of the protein is coupled to an engineered pheromone sensing system based on the FUS1 promoter present in α-type <i>S. cerevisiae</i>.
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             Myrosinase is introduced into <i>S. boulardii</i>, it is an enzyme that catalyzes the reaction of glucosinolate (present in cruciferous vegetables) to the chemopreventive sulphoraphane. Its expression is coupled to an engineered pheromone sensing system to limit its production to yeast accumulating around tumors.
 
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Revision as of 08:35, 17 October 2018

Interlab - iGEM Chalmers-Gothenburg 2018

Project Description

Attachment

Our engineered yeast adheres to the membrane protein Syndecan1 on the cancer cell surface. To do so, a protein complex is introduced consisting of Histone Like Protein A (HlpA), which specifically binds to Syndecan1, a linker, and the yeast cell surface display protein Aga2.

Yeast attaching on cancer cells
Yeast attaching on cancer cells

Detection

S. boulardii produces ultrasound-detectable gas vesicles, a mixed gene-set from prokaryotic gas vesicle operons. To allow single transcript expression in a eukaryote, 2A viral peptide sequences that cleave themselves are introduced between the genes.

Treatment

Myrosinase is introduced into S. boulardii, it is an enzyme that catalyzes the reaction of glucosinolate (present in cruciferous vegetables) to the chemopreventive sulphoraphane. Its expression is coupled to an engineered pheromone sensing system to limit its production to yeast accumulating around tumors.

Yeast attaching on cancer cells