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interfere with injured parts of the skin. Even though the risk of infection with | interfere with injured parts of the skin. Even though the risk of infection with | ||
cultured <span style="font-style: italic;">Plasmodium</span> that is not in its vector, the <span style="font-style: italic;">Anopheles</span> fly | cultured <span style="font-style: italic;">Plasmodium</span> that is not in its vector, the <span style="font-style: italic;">Anopheles</span> fly | ||
− | (and therefore an S2 organism <sup><a href="#safety_refs">1</a></sup>) is very low it still occurred in some cases | + | (and therefore an S2 organism <sup><a href="#safety_refs">1</a></sup>) |
− | we intended to avoid working with the parasite itself. | + | is very low it still occurred in some cases<sup><a href="#safety_refs">2</a></sup> |
+ | , and we intended to avoid working with the parasite itself. | ||
Instead, we chose to work only with genomic DNA extracted from cultured <span style="font-style: italic;">Plasmodium</span> parasites. | Instead, we chose to work only with genomic DNA extracted from cultured <span style="font-style: italic;">Plasmodium</span> parasites. | ||
We did not perform these extractions by ourselves but asked laboratories which are | We did not perform these extractions by ourselves but asked laboratories which are | ||
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<sup>2</sup> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88999/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88999/</a> | <sup>2</sup> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88999/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88999/</a> | ||
</div> | </div> | ||
− | |||
Latest revision as of 08:44, 17 October 2018
Safety
Finding out if our primers can not only detect synthetic DNA oligos but also DNA
of real Plasmodium parasites made us realise that we actually might have to work
with an organism that could potentially cause disease. Malaria is an infectious
disease and handling Plasmodium parasites poses the risk of infection when they
interfere with injured parts of the skin. Even though the risk of infection with
cultured Plasmodium that is not in its vector, the Anopheles fly
(and therefore an S2 organism 1)
is very low it still occurred in some cases2
, and we intended to avoid working with the parasite itself.
Instead, we chose to work only with genomic DNA extracted from cultured Plasmodium parasites.
We did not perform these extractions by ourselves but asked laboratories which are
culturing Plasmodium parasites to extract the DNA and provide it to us.
By collaborating with these labs we avoided getting in touch with the parasite
itself and could conduct our experiments on S1 level.
List of References
1 https://www.baua.de/DE/Angebote/Rechtstexte-und-Technische-Regeln/Regelwerk/TRBA/pdf/TRBA-100.pdf?__blob=publicationFile, page 462 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88999/