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Revision as of 23:08, 28 June 2018
Methnote
Project Description
Our aim is to engineer a biosensor, named MethNote, that is capable of sensing the greenhouse gas, Methane, in the local environment. We plan to combine two previous approaches, namely, conversion of methane to methanol using the enzyme complex methane monooxygenase (sMMO genes from the bacteria M. Capsulatus), and subsequent methanol detection, via the AOX promoter fused with RFP, in a methylotrophic yeast chassis(Pichia pastoris).
Given Methane's significant role in causing climate change by being far more effective than CO2, and its substantial rise in concentrations over the years, it has become an important issue to be dealt with keeping environment and public health into perspective. We envision our biosensor's industrial usage for in-situ detection of methane concentrations such as identification of methane pipe leaks and localized atmospheric methane concentrations in regions of substantial emissions like cattle farms, wetlands etc.
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