Difference between revisions of "Team:Aix-Marseille/Composite Part"
(→BBa_K2718006) |
(→BBa_K2718006) |
||
| Line 13: | Line 13: | ||
=BBa_K2718006= | =BBa_K2718006= | ||
| − | Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). [http://parts.igem.org/Part:BBa_K2718022 BBa_K2718005] is in RFC 10, and part comes from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300].To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [https://www.dovepress.com/activities-of-methionine-gamma-lyase-in-the-acidophilic-archaeon-ldquo-peer-reviewed-article-RRB]. | + | Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). [http://parts.igem.org/Part:BBa_K2718022 BBa_K2718005] is in RFC 10, and part comes from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300]. Promotor is [http://parts.igem.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], it's IPTG inducible promotor. To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [https://www.dovepress.com/activities-of-methionine-gamma-lyase-in-the-acidophilic-archaeon-ldquo-peer-reviewed-article-RRB]. |
https://static.igem.org/mediawiki/parts/1/16/--Aix-Marseille--Shcema_MGL_parts.jpg | https://static.igem.org/mediawiki/parts/1/16/--Aix-Marseille--Shcema_MGL_parts.jpg | ||
Revision as of 13:39, 17 October 2018
Composite Parts
Composite Parts
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. BBa_I13507 is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
BBa_K2718006
Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). BBa_K2718005 is in RFC 10, and part comes from BBa_K1493300. Promotor is BBa_R0011, it's IPTG inducible promotor. To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [1].
