Introduction

We aim to provide a novel chassis with improved bio-safety capabilities. This will take the form of a Maxicell - achromosomal E. coli - which is incapable of reproducing or of performing horizontal gene transfer. In order to prevent horizontal gene transfer we intend to take a two pronged approach; Semantic Containment will mean that other organisms should not be able to 'read' the DNA of the Maxicell if they should take it up and a DNA degrading killswitch will break up the Maxicell plasmid/s after a given time span.

DNA Degradation Switch Concept

The implementation of our DNA degradation system requires 2 constructs:

• Colicin E2 plasmid (pCol) - Colicin E2 is a nicking endonuclease which acts without specificity to cut both single and double stranded DNA. This plasmid will contain Constitutive Anderson Promoter, RBS, Colicin E2 coding sequence and T1 Terminator.
• Immunity Plasmid - Imm2 is the immunity protein for Colicin E2. Imm2 binds to Colicin E2 with high affinity and when present in equimolar concentration prevents its DNase activity. This plasmid will contain Constitutive Anderson Promoter, RBS, Imm2 Coding Sequence and T1 Terminator. In addition a site for the homing endonuclease I-SceI will be present that when cut turns off Imm2 expression.

Prior to Maxicell formation our E. coli will be transformed with pImm, this will cause a build up of Imm2 within the cell. At this phase of the model only equations for Imm2 synthesis are evaluated.

Once Imm2 concentration has reached equilibrium the E. coli can then be transformed with pCol. Colicin E2 will be expressed within the cell however the build-up of Imm2 and its continued expression ensure it remains inactive. At this phase in the model all equations are evaluated.

In order to break up the chromosome of our E. coli and form Maxicells we have obtained a strain from the Leach Lab containing a number of I-SceI sites within the chromosome. When I-SceI is introduced and Maxicells are formed pImm will also be cut 'turning off' expression of Imm2. At this phase in the model all equations are evaluated however rate of imm2 transcription is 0 and halflives for Imm2 and Colicin E2 are altered to reflect that replication is no longer taking place.

By modeling this simple killswitch we can determine the plausibility of the system as well as screen across different Anderson Promoters and RBS to find the killswitch activation time closest to the length of time for which Maxicells can continue expressing protein.

Mass Action Equations

Mass Action Equations are commonly used to represent chemical reactions and provide a starting point for mechanistic modelling of a variety of phenomena. The laws of mass action state that the rate of any chemical reaction is proportional to the product of the masses of the reacting substances, with each mass raised to a power equal to the coefficient that occurs in the chemical equation [2]. The mass action equations in Figure 1 can be used to represent protein expression when taking into account the effect of ribosome binding sites and promoters:

Ordinary Differential Equations

The model uses a simple set of ordinary differential equations derived from mass action equations:

p_imm and p_col - Promoter Strength - The Anderson Promoter Collection [1] consists of 19 constitutive promoters that have been characterized by their relative strengths in E.coli.

tr_imm - Rate of Imm Transcription - Transcription proceeds at an average speed of 60 nucleotides per second [2]. Imm is 258 nucleotides in length [3] giving transcription rate of 60/258. In Maxicells imm is not transcribed and hence this rate is set to 0.

deg_mRNA - Rate of mRNA Degradation - Average mRNA halflife in E. coli is 5 minutes [4]. deg_mRNA = ln(2)/halflife [4].

tr_col - Rate of Col Transcription - Transcription proceeds at an average speed of 60 nucleotides per second [2]. Col is 1743 nucleotides in length [5] giving transcription rate of 60/581.

rbs_imm and rbs_col - RBS Strength - The Community RBS Collection [6] consists of 3 ribosome binding sites that have been characterized by their relative strengths in E.coli.

trl_imm - Rate of Imm2 Translation - Translation proceeds at an average speed of 20 amino acids per second [2]. Imm2 is 83 amino acids in length [3] giving translation rate of 20/86.

deg_imm - Rate of Imm2 Degradation - in replicating E.coli effective halflife is the time taken for a generation approximately 20 minutes. In Maxicells halflife was determined using ProtParam Tool [7]. ProtParam uses the N-end rule to determine protein halflife, the estimates given for Imm2 are >10hrs in E.coli and 30hrs in reticulocytes hence an average 20hr halflife is used. deg_imm = ln(2)/halflife [4]

trl_col - Rate of Colicin E2 Translation - Translation proceeds at an average speed of 20 amino acids per second [2]. Colicin E2 is 581 amino acids in length [5] giving translation rate of 20/581.

deg_col - Rate of Colicin E2 Degradation - in replicating E.coli effective halflife is the time taken for a generation approximately 20 minutes. In Maxicells halflife was determined using ProtParam Tool [7]. ProtParam uses the N-end rule to determine protein halflife, the estimates given for Colicin E2 are >10hrs in E.coli and 30hrs in reticulocytes hence an average 20hr halflife is used. deg_col = ln(2)/halflife [4]

Results

Figure 2 demonstrates the results obtained from an initial non-screening run to investigate the plausibility of the killswitch design. Results indicate that free Colicin E2 will be present after 19.6 hours which is promising given the range of promoters and ribosome binding sites over which to screen. Quantities given of Imm2 mRNA, Colicin E2 mRNA, Imm2 and Colicin E2 are presented in relative units.

The results from this initial run are characteristic of those obtained when screening over Anderson Promoters and Ribosome Binding Sites. With only pImm transformed (left of first red line) we observe Imm2 mRNA very rapidly reaching equilibrium and Imm2 also reaching equilibrium shortly after. pCol is transformed (between first and second red lines) and reaches a much lower equilibirum at a slower rate compared to Imm2 due its larger size and hence slower rates of transcription (of Colicin E2 mRNA) and translation. Maxicell formation occurs (at second red line) and pImm is as a result linearized, we observe an immediate spike in Imm2 production due to the now longer halflife of Imm2 when in non-dividing Maxicells however this quickly drops as no new Imm2 mRNA is produced. We can also observe considerably higher Colicin E2 expression once maxicells are formed which is again due the longer halflife of proteins when not being diluted due to cell division. Free Colicin E2 becomes present at 19.6 hours and hence DNA degradation occurs and maxicells are deactivated.

Figure 3 displays the distribution of different killswitch activation times obtained by screening across Anderson Promoters and Ribosome Binding Sites. We can observe that there is a wide variation in activation times between 1.8 minutes and 194 hours interestingly we can also observe that the distribution closely approximates a power law distribution with fast activation time (0-50 hours) being significatly more numerous than those with longer activation times (>50 hours)

Figure 4 displays the protein levels over time for runs 20, 457 and 2585 which correspond to the slowest, fastest and average activation times of 194 hours, 0.03 hours (1.8 minutes) and 33.28 hours.

Sensitivity Analysis

Table 1 below summarises the average change in Degradation Switch Activation Time per 1% change in paramter value.

Table 2 (below) gives the Total Order FAST indices.

Conclusions

Extended Model

Contact EdiGEM18

Feel free to leave us a comment on social media!

• Twitter
• Facebook