Difference between revisions of "Team:Uppsala/test10"

Line 10: Line 10:
  
 
<html>
 
<html>
<head>
+
<div class="body">
<link href='https://fonts.googleapis.com/css?family=Open+Sans|Oswald:300' rel='stylesheet' type='text/css'>
+
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.4.0/css/font-awesome.min.css">
+
<script src="http://instafeedjs.com/js/instafeed.min.js" > </script>
+
  
<!-- TODO: ADD FOOTER AND NAV -->
 
  
 +
<head>
 +
<meta content="text/html; charset=utf-8" http-equiv="Content-Type">
 +
<meta content="width=device-width, initial-scale=1" name="viewport">
 +
<meta content="IE=edge" http-equiv="X-UA-Compatible">
 +
<meta name="x-apple-disable-message-reformatting">
 +
<title>National Geographic | PLANET OR PLASTIC?</title>
 +
<link href="https://s3.amazonaws.com/temp-natgeo-geograph-fonts/ngp/geograph.css" rel="stylesheet">
 +
<style type="text/css">
  
<!-- <meta name="viewport" content="width=device-width, initial-scale=1"> -->
+
                                                                /* CLIENT STYLES */
 +
                                                                .ReadMsgBody{width:100%;} .ExternalClass{width:100%;} /* Force Hotmail to display emails at full width */
 +
                                                                .ExternalClass, .ExternalClass p, .ExternalClass span, .ExternalClass font, .ExternalClass td, .ExternalClass div {line-height: 100%;} /* Force Hotmail to display normal line spacing */
 +
                                                                body, table, td, a{-webkit-text-size-adjust:100%; -ms-text-size-adjust:100%;} /* Prevent WebKit and Windows mobile changing default text sizes */
 +
                                                                table, td{mso-table-lspace:0pt; mso-table-rspace:0pt;} /* Remove spacing between tables in Outlook 2007 and up */
 +
                                                                img{-ms-interpolation-mode:bicubic;} /* Allow smoother rendering of resized image in Internet Explorer */
 +
       
 +
                                                                /* RESET STYLES */
 +
                                                                body{margin:0; padding:0;}
 +
                                                                img{border:0; height:auto; line-height:100%; outline:none; text-decoration:none;}
 +
                                                                table{border-collapse:collapse !important;}
 +
                                                                body{height:100% !important; margin:0; padding:0; width:100% !important;}
 +
                                                                a {text-decoration: none;}
 +
                                                                a:visited {color:#c0c0c0; text-decoration:none;}
 +
                                             
 +
                                        [style*="GeoBrandBlack"] {
 +
                                          font-family:'GeoBrandBlack', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
        [style*="GeoBrandBold"] {
 +
                                          font-family:'GeoBrandBold', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
          [style*="GeoBrandRegular"] {
 +
                                          font-family:'GeoBrandRegular', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
       
 +
        [style*="GeoEditBold"] {
 +
                                          font-family:'GeoEditBold', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
        [style*="GeoEditMedium"] {
 +
                                          font-family:'GeoEditMedium', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
        [style*="GeoEditRegular"] {
 +
                                          font-family:'GeoEditRegular', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
       
 +
        [style*="GeoEditRegularItalic"] {
 +
                                          font-family:'GeoEditRegularItalic', CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif !important;
 +
                                      }
 +
                                         
  
 +
                                                   
  
<style type="text/css">
+
                                                                @media screen and (max-width: 640px) {   
 +
                                                               
 +
                                                                    .hide {display:none !important;}
 +
                                                                    .center { text-align:center !important;}
 +
                                                                    .mobile-only {display: block !important; max-height: none !important; max-width: 640px !important; visibility: visible !important;}
  
 +
                                                                    .responsivetable{
 +
                                                                        width:100% !important;}
 +
                                                                   
 +
                                                                      .responsivetable70{
 +
                                                                        width:70% !important;}
 +
                                                                   
 +
                                                                    .responsivetable80{
 +
                                                                        width:80% !important;}
 +
                                                                   
 +
                                                                    .responsivetable90{
 +
                                                                        width:90% !important;}
 +
                                                                   
 +
                                                                    .full_img {
 +
                                                                        width: 100% !important;}
 +
                                                                 
 +
                                                                    .newrow {
 +
                                                                        display: block !important;
 +
                                                                        width: 100% !important;}
  
 +
                                                                    .centered {
 +
                                                                        text-align: center !important;}
 +
                   
 +
                                                                    .nopadding {
 +
                                                                        padding: 0px !important;}
 +
                                                                   
 +
                                                                    .noborder {
 +
                                                                          border: 0px !important;}
 +
                                                                   
 +
                                                                    .paddingtop20 {
 +
                                                                          padding: 20px 0px 0px 0px !important;}
 +
                                                                       
 +
                                                                        .paddingtop5 {
 +
                                                                          padding: 5px 0px 0px 0px !important;}
 +
                                                                       
 +
                                                                          .paddingbottom30 {
 +
                                                                          padding: 0px 0px 30px 0px !important;}
 +
                                                                   
 +
                                                                   
 +
                                                                    .subheropadding {
 +
                                                                          padding: 50px 0px 0px 0px !important;}
 +
                                                                       
 +
                                                                          .intropadding {
 +
                                                                        padding: 35px 0px 40px 0px !important;}
 +
                                                                   
 +
                                                       
 +
                                                                    .headlinefont {
 +
                                                                        font-size: 24px !important;
 +
                                                                        line-height: 28px !important;
 +
                                                                            letter-spacing: .22px !important;
 +
                                                                            }
 +
                                                                       
 +
                                                                        .mappingwidthimg {
 +
                                                                            width: 60px !important;}
 +
                                                                       
 +
                                                                                    .iconwidth{
 +
                                                                                      width:45% !important;
 +
                                                                                      height: auto !important;
 +
                                                      }
 +
                                                                       
 +
                                                                   
  
 +
                                                                }
 +
                                   
 +
                                                       
 +
                                                         
 +
                                                                @media screen and (max-width: 500px) {
 +
                                                         
 +
                                         
 +
                                                                       
 +
                                                             
  
 +
                                                          .imageswap {
 +
                                                          background: #000000 url("https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/Plastic_20180516_mobile-500px_b.jpg")
 +
                                                          center/cover !important;
 +
                                                          text-align: center !important;
 +
                                                          height: 294px !important;
 +
                                                      }
 +
                                                      .imageswaprecovery {
 +
                                                background: #000000 url("https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/20150516-footer-mobile-Recovery.jpg") no-repeat !important;
 +
                                                background-size: 500px 400px !important;
 +
                                                text-align: center !important;
 +
                                                height: 400px !important;
 +
                                            }
  
 +
                                                          .mobilewidth{
 +
                                                          width:80% !important;
 +
                                                          height: auto !important;
 +
                                                      }
  
 +
                                                   
 +
                                             
 +
                                                .mobileheaderpadding{
 +
                                                          padding-bottom:50px !important;
 +
                                                         
 +
                                                      }
 +
                                               
 +
                                                      }
 +
       
 +
        @media screen and (max-width: 425px) {
 +
                                                           
 +
                                                         
 +
                                                           
 +
                                                            .full_imgtitle {
 +
                                                                            width: 100% !important;}
 +
                                                           
 +
      }
  
  
@media only screen and (max-width: 800px) {
 
    #toc{
 
        display: none;
 
    }
 
    .content-text{
 
    margin-right:50vw;
 
    }
 
}
 
  
  
 +
</style><!--[if gte mso 9]>
 +
<xml>
 +
<o:OfficeDocumentSettings>
 +
<o:AllowPNG/>
 +
<o:PixelsPerInch>96</o:PixelsPerInch>
 +
</o:OfficeDocumentSettings>
 +
</xml>
 +
<![endif]-->
 +
<!--[if gt mso 15]>
 +
    <style type="text/css" media="all">
 +
    /* Outlook 2016 Height Fix */
 +
    table, tr, td {border-collapse: collapse;}
 +
    tr { font-size:0px; line-height:0px; border-collapse: collapse; }
 +
    </style>
 +
    <![endif]-->
 +
   
 +
<!--[if (gte mso 9)]>
 +
<style type="text/css">
 +
..msotextlink {text-decoration: underline !important;}
 +
</style>
 +
<![endif]-->
  
 +
<style type="text/css">
 +
                                          /* iOS BLUE LINKS */
 +
                                   
 +
                                    a[x-apple-data-detectors]
 +
                                    {
 +
                                    color: inherit !important;
 +
                                    text-decoration: none !important;
 +
                                    font-size: inherit !important;
 +
                                    font-family: inherit !important;
 +
                                    font-weight: inherit !important;
 +
                                    line-height: inherit !important;
 +
                                    }
 +
                     
 +
</style>
 +
<style type="text/css">
 +
        /* adds Nat Geo yellow bg color */
 +
.textlinkhover:hover  {
 +
background-color: #ffcc00 !important;
 +
transition: all .125s ease-in !important;
 +
            color: #000000 !important;
 +
}               
 +
    </style>
 +
<!--[if (gte mso 9)]>
 +
<style type="text/css">
 +
.msotextlink {text-decoration: underline !important;}
 +
</style>
 +
<![endif]-->
  
 
 
 
 
 
 
html, body {
 
  height: 100%;
 
}
 
 
body {
 
  font: 11px "Open Sans", sans-serif;
 
  -webkit-font-smoothing: antialiased;
 
  -moz-osx-font-smoothing: grayscale;
 
  margin: auto;
 
  display: flex;
 
  flex-flow: column nowrap;
 
  justify-content: space-between;
 
}
 
 
ul {
 
  list-style: none;
 
}
 
 
a {
 
  text-decoration: none;
 
}
 
 
.generic-anchor {
 
  color: #8DB9ED;
 
}
 
.generic-anchor:visited {
 
  color: #8DB9ED;
 
}
 
.generic-anchor:hover {
 
  color: #ccc;
 
}
 
 
.flex-rw {
 
  display: flex;
 
  flex-flow: row wrap;
 
}
 
 
 
footer {
 
  background: #373737;
 
  margin-top: auto;
 
  width: 100%;
 
}
 
 
.footer-list-top {
 
  width: 33.333%;
 
}
 
 
.footer-list-top > li {
 
  text-align: center;
 
  padding-bottom: 10px;
 
}
 
 
.footer-list-header {
 
  padding: 10px 0 5px 0;
 
  color: #fff;
 
  font: 2.3vw "Oswald", sans-serif;
 
}
 
 
.footer-list-anchor {
 
  font: 1.3em "Open Sans", sans-serif;
 
}
 
 
.footer-social-section {
 
  width: 100%;
 
  align-items: center;
 
  justify-content: space-around;
 
  position: relative;
 
  margin-top: 5px;
 
}
 
 
.footer-social-section::after {
 
  content: "";
 
  position: absolute;
 
  z-index: 1;
 
  top: 50%;
 
  left: 10px;
 
  border-top: 1px solid #ccc;
 
  width: calc(100% - 20px);
 
}
 
 
.footer-social-overlap {
 
  position: relative;
 
  z-index: 2;
 
  background: #373737;
 
  padding: 0 20px;
 
}
 
 
.footer-social-connect {
 
  display: flex;
 
  align-items: center;
 
  font: 3.5em "Oswald", sans-serif;
 
  color: #fff;
 
}
 
 
.footer-social-small {
 
  font-size: 0.6em;
 
  padding: 0px 20px;
 
}
 
 
.footer-social-overlap > a {
 
  font-size: 3em;
 
}
 
 
.footer-social-overlap > a:not(:first-child) {
 
  margin-left: 0.38em;
 
}
 
 
.footer-bottom-section {
 
  width: 100%;
 
  padding: 10px;
 
  border-top: 1px solid #ccc;
 
  margin-top: 10px;
 
}
 
 
.footer-bottom-section > div:first-child {
 
  margin-right: auto;
 
}
 
 
.footer-bottom-wrapper {
 
  font-size: 1.5em;
 
  color: #fff;
 
}
 
 
.footer-address {
 
  display: inline;
 
  font-style: normal;
 
}
 
 
@media only screen and (max-width: 768px) {
 
  .footer-list-header {
 
    font-size: 2em;
 
  }
 
 
  .footer-list-anchor {
 
    font-size: 1.1em;
 
  }
 
 
  .footer-social-connect {
 
    font-size: 2.5em;
 
  }
 
 
  .footer-social-overlap > a {
 
    font-size: 2.24em;
 
  }
 
 
  .footer-bottom-wrapper {
 
    font-size: 1.3em;
 
  }
 
}
 
@media only screen and (max-width: 568px) {
 
  main {
 
    font-size: 5em;
 
  }
 
 
  .footer-list-top {
 
    width: 100%;
 
  }
 
 
  .footer-list-header {
 
    font-size: 3em;
 
  }
 
 
  .footer-list-anchor {
 
    font-size: 1.5em;
 
  }
 
 
  .footer-social-section {
 
    justify-content: center;
 
  }
 
 
  .footer-social-section::after {
 
    top: 25%;
 
  }
 
 
  .footer-social-connect {
 
    margin-bottom: 10px;
 
    padding: 0 10px;
 
  }
 
 
  .footer-social-overlap {
 
    display: flex;
 
    justify-content: center;
 
  }
 
 
  .footer-social-icons-wrapper {
 
    width: 100%;
 
    padding: 0;
 
  }
 
 
  .footer-social-overlap > a:not(:first-child) {
 
    margin-left: 20px;
 
  }
 
 
  .footer-bottom-section {
 
    padding: 0 5px 10px 5px;
 
  }
 
 
  .footer-bottom-wrapper {
 
    text-align: center;
 
    width: 100%;
 
    margin-top: 10px;
 
  }
 
}
 
@media only screen and (max-width: 480px) {
 
  .footer-social-overlap > a {
 
    margin: auto;
 
  }
 
 
  .footer-social-overlap > a:not(:first-child) {
 
    margin-left: 0;
 
  }
 
 
  .footer-bottom-rights {
 
    display: block;
 
  }
 
}
 
@media only screen and (max-width: 320px) {
 
  .footer-list-header {
 
    font-size: 2.2em;
 
  }
 
 
  .footer-list-anchor {
 
    font-size: 1.2em;
 
  }
 
 
  .footer-social-connect {
 
    font-size: 2.4em;
 
  }
 
 
  .footer-social-overlap > a {
 
    font-size: 2.24em;
 
  }
 
 
  .footer-bottom-wrapper {
 
    font-size: 1.3em;
 
  }
 
}
 
 
 
 
 
 
 
 
 
 
 
 
</style>
 
 
</head>
 
</head>
 +
<body style="margin: 0; padding: 0; -webkit-text-size-adjust: none; -ms-text-size-adjust: none;">
 +
<div style="display: none; font-size: 0px; line-height: 0px; max-height: 0px; max-width: 0px; width: 0px; opacity: 0; overflow: hidden;">
 +
Which one will you choose? &nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;‌&nbsp;&nbsp;
 +
</div>
 +
    <div class="hide" style="letter-spacing: 596px; line-height: 0; mso-hide: all">&nbsp;</div>
 +
    <!-- DESKOP BANNER -->
 +
<table bgcolor="#000000" border="0" cellpadding="0" cellspacing="0" class="hide" width="100%" style="min-width:100%;">
 +
<tbody><tr>
 +
<td align="center" style="padding-top: 12px; padding-bottom: 12px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 620px;" width="620">
 +
<tbody><tr>
 +
<td align="left" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandRegular'; font-size: 8px; line-height: 13px; color: #ffffff;"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDWA&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration:none; text-transform: uppercase; letter-spacing: 2.4px; color: #ffffff;" target="_blank">Choose the planet. Every action matters.</a></td>
 +
<td align="right" class="hide" data-content-region-name="SignInJoin" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandRegular'; font-size: 8px; line-height: 13px; letter-spacing: 2.4px; color: #ffffff;">
 +
<a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDWB&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration: none; text-transform: uppercase; color: #ffffff;" target="_blank">JOIN&nbsp;&nbsp;&nbsp;&nbsp;</a>|<a href="#" style="text-decoration: none; text-transform: uppercase; color: #ffffff;" target="_blank">&nbsp;&nbsp;&nbsp;&nbsp;VIEW ONLINE</a>
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
</tbody></table><!-- END DESKTOP BANNER -->
 +
<!-- MOBILE BANNER -->
 +
<!--[if !mso]><!-->
 +
<div class="mobile-only" style="font-size: 0; max-height: 0; overflow: hidden; display: none; mso-hide: all; min-width:100%;">
 +
<table bgcolor="#000000" border="0" cellpadding="0" cellspacing="0" width="100%">
 +
<tbody><tr>
 +
<td align="center" style="padding-top: 13px; padding-bottom: 13px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable90" style="width: 640px;" width="640">
 +
<tbody><tr>
 +
<td align="center" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandRegular'; font-size: 10px; line-height: 13px; color: #ffffff;"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDWA&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration: none; text-transform: uppercase; letter-spacing: 2px; color: #ffffff;" target="_blank">Choose the planet.</a></td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
</div><!--<![endif]-->
 +
<!-- END MOBILE BANNER -->
  
 +
<!-- HERO -->
 +
    <!--[if (gte mso 9)|(IE)]><table border="0" cellpadding="0" cellspacing="0" width="100%"><tr><td align="center"><table border="0" cellpadding="0" cellspacing="0" bgcolor="#ffffff" width="100%"><tr>
 +
<td width="1000" align="center" style="width: 1000px;padding: 50px 0px 50px 0px;"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDCW&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img src="https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/Plastic_20180516_hero_a_mso-1300px.jpg" width="1000" alt="" border="0"/></a></td>
 +
</tr></table></td></tr></table><![endif]-->
 +
    <!--[if gte mso 9]><!-->
 +
    <div style="mso-hide: all;">
 +
<table border="0" cellpadding="0" cellspacing="0" style="mso-hide: all;" width="100%">
 +
<tbody><tr>
 +
<td align="center" background="https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/Plastic_20180516_hero_1300px.jpg" bgcolor="#222222" class="imageswap" style="background: #222222 url(https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/Plastic_20180516_hero_1300px.jpg) center/cover; background-image: url(https://static.cdn.responsys.net/i5/responsysimages/content/natgeo/Plastic_20180516_hero_1300px.jpg); background-size: 100%; background-size: cover; padding: 0px 0px 0px 0px;" valign="bottom" width="100%" height="226">
 +
<div align="center" style="mso-hide: all;">
 +
<table align="center" border="0" cellpadding="0" cellspacing="0" style="max-width: 600px;" width="100%">
 +
<tbody>
 +
<tr>
 +
 +
<td align="center" style="padding: 50px 0px 130px 0px;" valign="top">
 +
<table align="center" valign="top" border="0" cellpadding="0" cellspacing="0" class="responsivetable80" style="width: 600px;" width="600">
 +
<tbody><tr>
 +
<td align="center">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 500px;" valign="middle" width="500">
 +
<tbody><tr>
 +
<td align="center" class="centered" style="padding-bottom:200px;">
 +
<a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDWD&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img align="absbottom" alt="National Geographic" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/header-logo-white.png" style="color: #ffffff; font-family:Helvetica,Arial,sans-serif;" width="150"></a>
 +
</td>
 +
</tr>
 +
                                                    <tr>
 +
<td align="center" class="centered mobileheaderpadding" style="padding-bottom:40px;">
 +
<a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYR&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img alt="PLANET OR PLASTIC?" class="mobilewidth" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/PoP_Tagline.png" style="color: #ffffff; font-family:Helvetica,Arial,sans-serif;" width="400"></a>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td align="center" style="padding-bottom:100px; font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoEditMedium'; font-size: 16px; line-height:24px; font-weight:normal; color:#ffffff; letter-spacing: 0.5;" width="350">National Geographic is embarking on a multi-year effort to raise awareness about the global plastic waste crisis. Learn what you can to do to help. <a class="msotextlink textlinkhover" href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYS&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="color: #ffffff; text-decoration: none; border-bottom: 2px solid #ffcc00;" target="_blank">Take your pledge</a>.
 +
</td>
 +
</tr>
 +
<tr>
 +
<td align="center" class="centered">
 +
<a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYR&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img alt="V" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/chevron-down.png" style="color: #ffffff; font-family:Helvetica,Arial,sans-serif;" width="37"></a>
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</div><!--[if gte mso 9]>
 +
        </v:textbox>
 +
      </v:rect>
 +
      <![endif]-->
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
        </div>
 +
    <!--<![endif]-->       
 +
  <!-- END HERO -->
 +
 +
<!-- Pledge -->
 +
<table border="0" cellpadding="0" cellspacing="0" width="100%" bgcolor="#000000" style="background-color: #000000;">
 +
<tbody><tr>
 +
<td align="center" style="padding-bottom:20px; padding-top:70px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 640px;" width="640">
 +
<tbody><tr>
 +
<td align="center"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYT&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img alt="" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/pop-pledge-logo.png" style="color: #ffffff; font-family:Helvetica,Arial,sans-serif;" width="125"></a></td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td align="center" style="padding-bottom:70px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable80" style="width: 500px;" width="500">
 +
<tbody><tr>
 +
<td align="center" style="padding-bottom: 20px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 500px;" width="500">
 +
<tbody><tr>
 +
<td align="center">
 +
<table border="0" cellpadding="0" cellspacing="0">
 +
<tbody><tr>
 +
<td align="center" class="headlinefont" style="font-family: Helvetica,Arial,sans-serif,'GeoBrandBold'; font-size: 32px; line-height: 38px; letter-spacing: 2px; padding: 5px 0px 0px 0px; font-weight: normal; color: #ffcc00; "><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYU&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration: none; color: #ffcc00;" target="_blank">CHOOSE THE PLANET.<br>EVERY ACTION MATTERS.</a></td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
</tbody></table>
 +
</td>
 +
</tr>
 +
                    <tr>
 +
<td align="center">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 500px;" width="500">
 +
<tbody><tr>
 +
<td align="center" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoEditMedium'; font-size: 16px; line-height:24px; color:#ffffff; letter-spacing: .5px;">Start today. Share your choice to reduce single-use plastic.</td>
 +
                            </tr>
 +
                                </tbody></table>
 +
                            </td>
 +
</tr>
 +
<tr>
 +
<td class="centered" style="padding-top: 25px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 600px;" width="600">
 +
<tbody><tr>
 +
<td align="center" class="newrow centered" style="padding-bottom:30px;">
 +
<table border="0" cellpadding="0" cellspacing="0" class="responsivetable">
 +
<tbody><tr>
 +
<td align="center" class="centered">
 +
<table align="center" bgcolor="#FFCC00" border="0" cellpadding="0" cellspacing="0" style="width: 240px;" width="240">
 +
<tbody><tr>
 +
<td align="center" height="35" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandBold'; font-size: 12px; line-height:15px; color:#000000;" valign="center"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYW&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="color: #000000; text-decoration: none; display: block; width: 100%; padding-top: 13px; padding-bottom: 13px; text-transform: uppercase; letter-spacing: 2px;" target="_blank">Take Your Pledge</a></td>
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<td align="right"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYY&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_aTnKShIAdj-zs7hMNpszfV4." target="_blank"><img alt="F" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/share_fb_white.png" style="color: #ffffff; font-family:Helvetica,Arial,sans-serif;" width="30.5"></a></td>
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<td align="right"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYA&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_aTnKShIAdj-zs7hMNpszfV4." target="_blank"><img alt="T" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/share_twitter_white.png" style="color: #000000; font-family:Helvetica,Arial,sans-serif;" width="31"></a></td>
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</tbody></table><!-- END Pledge -->
  
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<table border="0" cellpadding="0" cellspacing="0" style="border-spacing: 0; mso-line-height-rule: exactly; mso-margin-bottom-alt: 0; mso-margin-top-alt: 0; mso-table-lspace: 0pt; mso-table-rspace: 0pt;" width="100%">
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<td align="left"><img align="absbottom" alt="" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/mapping-bottom.png" style="display: block;" width="300"></td>
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<table border="0" cellpadding="0" cellspacing="0" width="100%">
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<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 640px;" width="640">
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<td align="center" class="sectionheadfont" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandBlack'; font-size: 42px; line-height: 32px; color: #cccccc; text-transform: uppercase; letter-spacing: 5px;">FROM THE MAGAZINE</td>
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</tbody></table><!-- END SECTION HEADER -->
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<table border="0" cellpadding="0" cellspacing="0" class="responsivetable" style="width: 640px;" width="640">
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<tbody><tr>
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<td align="center"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYB&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." target="_blank"><img alt="" class="full_img" src="https://static.cdn.responsys.net/i5/responsysimages/natgeo/contentlibrary/201805/plastic_20180516/20180516_hero1.jpg" style="color: #000000; font-family:Helvetica,Arial,sans-serif;" width="640"></a></td>
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<tbody><tr>
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<td style="padding-bottom: 15px;">
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<td align="left" height="23" style="font-family: CenturyGothic, AppleGothic, Gill Sans, Gill Sans MT, sans-serif,'GeoBrandRegular'; font-size: 12px; line-height: 15px; mso-border-left-alt: 3px solid #ffcc00; mso-border-right-alt: 3px solid #ffcc00;"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYC&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration: none; text-transform: uppercase; letter-spacing: 3px; color: #000000; border-left: 3px solid #ffcc00; border-right: 3px solid #ffc000;" target="_blank">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENVIRONMENT&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a></td>
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<td align="left" class="headlinefont" style="font-family: Helvetica,Arial,sans-serif,'GeoEditBold'; font-size: 28px; line-height: 34px; letter-spacing: .3px; padding: 5px 0px 0px 0px; font-weight: normal;"><a href="https://e.nationalgeographic.com/pub/cc?_ri_=X0Gzc2X%3DYQpglLjHJlYQGu4sjD2zb80LDDjiY0lftwGuszglYw8wzdlY1zdBKft9qkzf4qBGFUCbzfVXtpKX%3DSRUABDYD&amp;_ei_=Eq2tf9zs59idfPO1Sc_9BbmFpTg2e5ywpTmvCBY5wxf_ApiS1fyT3AsZo-dtALImyWGOneUjM6riFaCMP3EFMaEiDGcR3VK_." style="text-decoration: none; color: #000000;" target="_blank">We Made It. We Depend on It. We're Drowning in It.</a></td>
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</tbody></table><!-- END FEATURE 1 -->
  
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        <div class="under-heading"><h1 id="Project_Description">Uppsala iGEM 2018</h1></div>
 
        <div class="igem-icon"><a href="https://2018.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2018/b/b0/T--Uppsala--graylogo.png"></a></div>
 
 
 
 
 
 
 
 
 
 
 
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                <li class="toclevel tocsection"><a href="#Project_Description" class="scroll"> <span id="whereYouAre"> Project Description  </span> </a>
 
                        <ul>
 
                            <li class="toclevel nav-item active"><a href="#top" class="nav-link scroll"> Overview </a></li>
 
                            <li class="toclevel nav-item"><a href="#Problem" class="nav-link scroll">  Problem  </a></li>
 
                            <li class="toclevel nav-item"><a href="#Solution" class="nav-link scroll">  Solution </a></li>
 
                            <li class="toclevel nav-item"><a href="#References" class="nav-link scroll"> References </a></li>
 
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                <p id="Project_Description">Nematode parasites of the strongyle family cause the agricultural industry substantial losses and grief each year due to the detrimental effects they have on livestock. Common issues include severe health damage in the host animal as well as resistance development to anthelmintics in the most commonly occurring strongyles. There are currently no easy methods for diagnosing these parasites. By reprogramming a smart bacterium to detect and report the presence of the parasites, we aim to develop a simple diagnostic method. This will provide the tools necessary to help farmers both to make decisions on whether to treat their animals and to prevent infection to begin with. </p>
 
 
 
 
           
 
 
 
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                    <div class="content-card-heading"><h1 id="top">Our Targets:</h1></div>
 
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                        <div class="inner-card left-card">
 
                            <h2>Small Strongyles</h2>
 
                            <br>
 
                            <div class="inner-card-text">
 
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                                <p>Small strongyles <i>(Cyathostominae)</i> are among the most common equine parasites with more than 52 species in their family. Horses are exposed when they graze on infested pastures, where they consume the worms in their larval stage. The strongyles move to the horses’ intestines, gathering into cysts in the intestinal wall and usually reach very large numbers.  These cysts eventually burst and the developed worms moves up towards the intestinal lumen where they become adult worms. The release of the larvae from the cysts can lead to lesions, diarrhea, and potential weight loss in the animal, a condition called cyathostominosis. When untreated, the mortality rate of a small strongyle infection can reach up to 50%. </p>
 
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                            <h2>Large Strongyles</h2>
 
                            <br>
 
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                                <p><i>Strongylus vulgaris</i> is the most pathogenic parasite in horses, posing a significant threat to the health of the animal. They, like small strongyles, live in the grass and infect the horse after being ingested. During the larval stages inside the animal, the parasite enters the intestinal blood vessels as a part of their life cycle. This causes them to commonly avoid deworming and detection measures. When the worms migrate in the arteries they cause inflammation in the arterial wall and induce the formation of blood clots. These blood clots may travel in the blood vessels and block smaller passages, inhibiting oxygen and nutrient supply to the surrounding tissues, and may result in colic or in the worst case, death.  </p>
 
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                            <img class="content-card-img" src="https://static.igem.org/mediawiki/2018/d/d6/T--Uppsala--Large.jpg">
 
 
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                <h1 id="Problem">The Worm Busters</h1>
 
               
 
                <p>One approach for designing a diagnostic system against the strongyles is to engineer a “smart” bacterium which will live in the intestinal tract and is capable of reacting to the presence of the parasite by emitting a quantifiable signal. This biosensor may, for instance, induce the production of a detectable fluorescent protein in the feces of the animal. Large strongyles, however, are more elusive and are less frequently present in the intestine during an infection. Here it would be suitable to instead try and detect the presence of the parasite on the pastures as to avoid infection altogether, by developing bacteria responding to the parasite outside of the animal body.  </p>
 
                    <br>
 
 
                <p> Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
                <p  > asdasd </p>
 
 
 
 
 
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
                  <h1 id="References">The Worm Busters</h1>
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
                <br>
 
                <p > Our work is dependent on finding one or more genes in <i>E.coli</i> which will, exclusively, be highly expressed when the cell is exposed to the parasitic worms. This is done by co-culturing <i>E.coli</i> in liquid medium along with live strongyles, which are harvested from feces and sterilized. From here, the E.coli cells are separated from the solution and their entire transcriptomic suite is extracted and sequenced to detect genes of interest. Any found genes which display promise will have to be validated by qPCR (which is a similar method) in a second run to confirm that they are only expressed due to the strongyle presence. Another approach to tackle our challenge is to screen for interaction between the surface proteins on the strongyle and short peptides. Through affinity screening of a random peptide library displayed on the surface of phages, we can select a peptide with a high affinity to the nematodes surface.  </p>
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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