Difference between revisions of "Team:Aix-Marseille/Basic Part"
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| − | Chitinase [http://parts.igem.org/Part:BBa_K2718020 BBa_K2718020]is a fungus (''Beauveria bassiana'') enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere to . Sequence comes from [https://www.uniprot.org/uniprot/Q8J1Y3 uniprot], we used peptidic sequence between 41 to 330 AA, that's correspond [https://www.ebi.ac.uk/interpro/protein/Q8J1Y3 catalitic domain]. We improve nucleotidic sequence with [https://eu.idtdna.com/CodonOpt IDT tool] for synthesis by ''E.coli''. Part is RFC 25 standards. We test activiy with Schales' procedure. | + | Chitinase [http://parts.igem.org/Part:BBa_K2718020 BBa_K2718020]is a fungus (''Beauveria bassiana'') enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere to . Sequence comes from [https://www.uniprot.org/uniprot/Q8J1Y3 uniprot], we used peptidic sequence between 41 to 330 AA, that's correspond [https://www.ebi.ac.uk/interpro/protein/Q8J1Y3 catalitic domain]. We improve nucleotidic sequence with [https://eu.idtdna.com/CodonOpt IDT tool] for synthesis by ''E.coli''. Part is RFC 25 standards. We test activiy with Schales' procedure [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975300/pdf/1754-6834-7-37.pdf]. |
=BBa_K2718021= | =BBa_K2718021= | ||
Revision as of 15:49, 17 October 2018
Basic Parts
Basic Parts
A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.
.
BBa_K2718005
Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). BBa_K2718005 is in RFC 10, and part comes from BBa_K1493300.To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [1].
BBa_K2718020
Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere to . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli. Part is RFC 25 standards. We test activiy with Schales' procedure [2].
BBa_K2718021
Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond to catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli, and there is his tag in C-ter. Part is RFC 25 standards. We test activiy with Schales' procedure.