Difference between revisions of "Team:Oxford/Collaborations"

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             <h2>Imperial College London iGEM - Wet Lab</h2>
 
             <h2>Imperial College London iGEM - Wet Lab</h2>
 
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<p>In conjunction with Imperial College, we tested a range of SoxS promoter strengths in order to create a functional library. GFP production was measured in response to hydrogen peroxide (and electrical stimuli on their part) to characterise a range of BLAST SoxS promoters. Our parts were to be assembled by the digestion of eCFP from the kit plate and the amplified promoter construct was digested using XbaI and EcoRI followed by ligation. A kit plate assay was performed to measure GFP expression as a function of both hydrogen peroxide and cell growth in order to determine the cytotoxic effects of the inducer.
 
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<p>Imperial constructed a promoter library using a BLAST search for the SoxS promoter which they characterised in response to an applied electrical current. To assist them, we characterised their parts in response to chemically induced oxidative stress. In return they provided us with a range of constructs which we could match to the promoter strength, predicted in our model to give the highest sensitivity to NO.  
 
<p>Imperial constructed a promoter library using a BLAST search for the SoxS promoter which they characterised in response to an applied electrical current. To assist them, we characterised their parts in response to chemically induced oxidative stress. In return they provided us with a range of constructs which we could match to the promoter strength, predicted in our model to give the highest sensitivity to NO.  
 
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Revision as of 16:51, 17 October 2018

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Collaborations

UK iGEM Meetup


On 12th and 13th July, we helped host the UK-wide iGEM meetup at Worcester College, Oxford in partnership with SynBio UK. Teams travelled from across the country to attend this two-day event that promoted collaboration, offered advice and gave an insight into what lies beyond the iGEM competition.

Imperial College London iGEM - Wet Lab


Imperial constructed a promoter library using a BLAST search for the SoxS promoter which they characterised in response to an applied electrical current. To assist them, we characterised their parts in response to chemically induced oxidative stress. In return they provided us with a range of constructs which we could match to the promoter strength, predicted in our model to give the highest sensitivity to NO.

While in our device SoxR is used to respond to NO, the short half-life prevents isolation for fluorescence assays. SoxR is not only activated by NO but any chemical which induces oxidative stress. Initially aiming to characterise the promoter in response to the superoxide generating herbicide, paraquat, we found that due to its toxicity, it was prohibited in the EU and required specialist clearance for research purposes. Hydrogen peroxide was chosen as a safer alternative which still produces reactive oxygen species in the cells, and thus activates SoxR.

Characterisation was two-fold. A plate reader assay was performed which measured fluorescence of the GFP from the promoter construct in response to 100uM hydrogen peroxide. In addition to this A600 (subscript 600) was measured, showing growth, which would allow determination of the cytotoxicity of hydrogen peroxide at levels required for induction of SoxR.

Imperial College London iGEM - Outreach


We teamed up with Imperial iGEM to hold a joint stall during New Scientist Live at the ExCel centre, London. Throughout the 4-day exhibition, which featured our respective project posters, we engaged with visitors to discuss various aspects of iGEM, genetic engineering, and our projects. In preparation for the event, we held weekly Skype meetings with the Imperial team in order to jointly design an educational leaflet designed to improve understanding about synthetic biology, including common laboratory techniques, to distribute to the general public. The leaflet is shown below:



Imperial College London iGEM - Software Testing


We tested a web app (‘let’s talk about this’) created by the Imperial iGEM team designed to help resolve team issues that may arise in extended projects. We provided feedback on various aspects of the software in order to improve its suitability as a tool to promote discussion.

METU HS iGEM - Mentoring


High School Mentorship

Tec Monterrey GDL iGEM - ?


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Tec Monterrey iGEM - ?


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Warwick iGEM - Modelling


Hill Functions