Difference between revisions of "Team:HBUT-China/Description"

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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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                    Description
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                        Wuhan China
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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                            In recent years, nickel ion pollution has become an environmental issue of public concern. China has stipulated that the maximum allowable concentration of nickel in surface water is 0.5mg/liter. The treatment of nickel ion in sewage today has certain limitations, such as high cost, low efficiency, and high energy consumption.
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                            Motivated by this, the HBUT-China team aimed to design recombinant strains for the detection of nickel ions using synthetic biology methods. First of all, we wanted to make an improvement on last year’s project. We replaced the fluorescent gene <i>mRFP</i>, whose protein requires excitation light to illuminate, with a bioluminescent gene <i>luxCDABE</i>. At the same time, the bioluminescent protein also solved a problem with interference from the natural fluorescence of the <i>E.coli</i>. Through modeling, we determined the most suitable linear range of nickel ion concentration.
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                            In the HP section, we did a nine-day water quality survey and learned about the public’s perception of nickel-ion contamination. We believe that we should not only improve nickel ion detection, but also focus on nickel ion absorbance/removal.
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                            By searching relevant sources, we found a Ni2+ channel protein gene,  <i>nikABCDE</i>. After inserting this gene into Nickel Hunter, upstream of our gene line, we found that it not only can absorb significantly more nickel ion, it also improved the accuracy of detection as the system becomes more sensitive to the amount of nickel ion.
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                            Next, we wanted to design a machine where we could put a sample into the its injection pool, then through the chip processing, a photoelectric conversion module, and a WiFi module, realize real-time monitoring of nickel ion levels on a mobile device.
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                            We were also concerned about the leakage of <i>E. coli</i> containing nickel ions into nature. We wanted to replace the chassis cells in yeast with two channel proteins, TjZNT1 and TgMTP1t2, on the cell membrane and tonoplast, which can transport nickel ions step by step, then nickel ions in the environment would accumulate in the vacuoles of these yeast cells, thereby reducing the concentration in the nickel ion environment. We wanted to then seek a means to filter out this Nickel ion-loaded yeast, thus removing or reducing nickel ion concentrations in the environment.
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Latest revision as of 17:17, 17 October 2018

Description

In recent years, nickel ion pollution has become an environmental issue of public concern. China has stipulated that the maximum allowable concentration of nickel in surface water is 0.5mg/liter. The treatment of nickel ion in sewage today has certain limitations, such as high cost, low efficiency, and high energy consumption.

Motivated by this, the HBUT-China team aimed to design recombinant strains for the detection of nickel ions using synthetic biology methods. First of all, we wanted to make an improvement on last year’s project. We replaced the fluorescent gene mRFP, whose protein requires excitation light to illuminate, with a bioluminescent gene luxCDABE. At the same time, the bioluminescent protein also solved a problem with interference from the natural fluorescence of the E.coli. Through modeling, we determined the most suitable linear range of nickel ion concentration.

In the HP section, we did a nine-day water quality survey and learned about the public’s perception of nickel-ion contamination. We believe that we should not only improve nickel ion detection, but also focus on nickel ion absorbance/removal.

By searching relevant sources, we found a Ni2+ channel protein gene, nikABCDE. After inserting this gene into Nickel Hunter, upstream of our gene line, we found that it not only can absorb significantly more nickel ion, it also improved the accuracy of detection as the system becomes more sensitive to the amount of nickel ion.

Next, we wanted to design a machine where we could put a sample into the its injection pool, then through the chip processing, a photoelectric conversion module, and a WiFi module, realize real-time monitoring of nickel ion levels on a mobile device.

We were also concerned about the leakage of E. coli containing nickel ions into nature. We wanted to replace the chassis cells in yeast with two channel proteins, TjZNT1 and TgMTP1t2, on the cell membrane and tonoplast, which can transport nickel ions step by step, then nickel ions in the environment would accumulate in the vacuoles of these yeast cells, thereby reducing the concentration in the nickel ion environment. We wanted to then seek a means to filter out this Nickel ion-loaded yeast, thus removing or reducing nickel ion concentrations in the environment.