Difference between revisions of "Team:Duke/Design"
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| − | <h2> | + | <h2>Modular Design and Path Optimization </h2> |
| − | <p><b>Maria</b> | + | <p><b>Maria</b> |
| + | The end goal of the project was to create a linear stretch of DNA containing all five genes in the pathway that could be recombineered into the E. coli genome. In creating this pathway, each gene was viewed as a separate "building block" that would be linked with the other blocks to create a complete pathway. Within one gene's "building block," there were several components: the 5' linker arm, the T7 promoter, the coding sequence of the gene, and the 3' linker arm. | ||
| + | </p> | ||
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| − | <h2>Promoter Optimization </h2> | + | <h2>Promoter Optimization Experimental Design </h2> |
| − | <p><b>Maria</b> | + | <p><b>Maria</b> The design of the following elements of the project was laid out, but not experimentally implemented. LINE BREAK HOW DO I DO THOSE. |
| − | + | Each of the 5 genes in the pathway was designed to be fitted with a different T7 bacteriophage promoter.</p> | |
Revision as of 19:05, 17 October 2018
Design
BAPT
Daniel
TAX10
Daniel
Modular Design and Path Optimization
Maria The end goal of the project was to create a linear stretch of DNA containing all five genes in the pathway that could be recombineered into the E. coli genome. In creating this pathway, each gene was viewed as a separate "building block" that would be linked with the other blocks to create a complete pathway. Within one gene's "building block," there were several components: the 5' linker arm, the T7 promoter, the coding sequence of the gene, and the 3' linker arm.
Promoter Optimization Experimental Design
Maria The design of the following elements of the project was laid out, but not experimentally implemented. LINE BREAK HOW DO I DO THOSE. Each of the 5 genes in the pathway was designed to be fitted with a different T7 bacteriophage promoter.
Design
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs