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We first got acquaintance with <a href="https://2018.igem.org/Team:Jilin_China/Collaborations" class="contentLink">Jilin_China</a> in Nankai Conference and kept contact with each other afterwards. To ensure the repeatability of our data, we sent C-terminal and N-terminal of ECFP to them, requesting them to test whether we have split the protein right. Meanwhile Jilin_China works on RNA thermosenser this year and they need to test the expression of sfGFP in different temperature, indicating in what condition the designated thermosensor is open. We gave a helping hand by examing wheather their positive control will change with different temperatures. As the result shows the floursence intensity of positive control has a linear correlation with temperature. | We first got acquaintance with <a href="https://2018.igem.org/Team:Jilin_China/Collaborations" class="contentLink">Jilin_China</a> in Nankai Conference and kept contact with each other afterwards. To ensure the repeatability of our data, we sent C-terminal and N-terminal of ECFP to them, requesting them to test whether we have split the protein right. Meanwhile Jilin_China works on RNA thermosenser this year and they need to test the expression of sfGFP in different temperature, indicating in what condition the designated thermosensor is open. We gave a helping hand by examing wheather their positive control will change with different temperatures. As the result shows the floursence intensity of positive control has a linear correlation with temperature. | ||
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+ | <div><span class="figureNum">Figure 1.</span>Fluorescence expression of positive control at different temperatures. The figure’s horizontal axis represents the temperature range (28℃ to 42℃), and the vertical axis represents fluorescence intensity of sfGFP. The red line is the sfGFP expression curve of NPC at 8th hours, the purple line is sfGFP expression curve of NPC at 6th hours, the blue line is sfGFP expression curve of MPC at 8th hours and the organe line is sfGFP expression curve of MPC at 6th hours.</div> | ||
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Revision as of 21:39, 17 October 2018
![](https://static.igem.org/mediawiki/2018/8/8c/T--Fudan-CHINA--Model_1.jpg)
Collaborations
"The essence of mathematics lies in its freedom."
![](https://static.igem.org/mediawiki/2018/e/ed/T--Fudan-CHINA--Fudanlogo.png)
We first got acquaintance with Jilin_China in Nankai Conference and kept contact with each other afterwards. To ensure the repeatability of our data, we sent C-terminal and N-terminal of ECFP to them, requesting them to test whether we have split the protein right. Meanwhile Jilin_China works on RNA thermosenser this year and they need to test the expression of sfGFP in different temperature, indicating in what condition the designated thermosensor is open. We gave a helping hand by examing wheather their positive control will change with different temperatures. As the result shows the floursence intensity of positive control has a linear correlation with temperature.
![](https://static.igem.org/mediawiki/2018/c/c0/T--Fudan-CHINA--JilinRe.png)
Figure 1.Fluorescence expression of positive control at different temperatures. The figure’s horizontal axis represents the temperature range (28℃ to 42℃), and the vertical axis represents fluorescence intensity of sfGFP. The red line is the sfGFP expression curve of NPC at 8th hours, the purple line is sfGFP expression curve of NPC at 6th hours, the blue line is sfGFP expression curve of MPC at 8th hours and the organe line is sfGFP expression curve of MPC at 6th hours.
![](https://static.igem.org/mediawiki/2018/5/5e/T--Fudan-CHINA--pek.jpg)
![](https://static.igem.org/mediawiki/2018/d/dd/T--Fudan-CHINA--tongji.jpg)
We decided to collect some interesting reasons for experiment failures and choose some of them to design a comic brochure, to add some amusement and at the same time remind everyone in the lab to be really careful and not to make mistakes during operation. Also we will distribute this brochure in our school and Giant Jamboree to get more researchers' attention in a humorous way. Luckily, we got response from iGEMers all over the world. Some of them are really miserable though bring us a lot of joy. We consider those stories lively but meaningful to all of us. Thus, thanks to our great talented drawer Hao Tian, a comic book was born — made up of funniest failure in lab, in a tearful smile. Read our comic now!
Thanks for support from: Fudan, ICT-Mumbai, NUDT_CHINA, JNFLS, Tongji-Software, TUST_China, GreatBay_China, SCU-China.
![](https://static.igem.org/mediawiki/2018/0/03/T--Fudan-CHINA--edg.jpg)
Exchanging ideas is always good for igemers and researchers, so we attended many meet-ups and conferences this year.
We intended to attend a conference in NKU on the 6th of July. However, bad weather condition prevented our flight. A video was made to introduce our project before we answered questions from professors and other iGEMers online. Advice was collected and applied to the refinement of project design and purpose. Also we watched the presentation of other teams, getting to know how their experiment goes on. We got familiar with Peking, NKU_CHINA and Jilin_China through this conference and developed collaboration afterwards.
![](https://static.igem.org/mediawiki/2018/5/52/T--Fudan-CHINA--itai.jpg)
![](https://static.igem.org/mediawiki/2018/0/0e/T--Fudan-CHINA--xcici.jpg)
Address
G604, School of Life Sciences, Fudan University
2005 Songhu Road, Yangpu, Shanghai, China
2005 Songhu Road, Yangpu, Shanghai, China
![](https://static.igem.org/mediawiki/2018/f/fc/T--Fudan-CHINA--teamBadgeWhite.png)