Difference between revisions of "Team:USP-Brazil/Demonstrate"

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           <div class="wet-content demonstrate">
 
           <div class="wet-content demonstrate">
 
             <h2><a name="demons">Demonstrate</a></h2>
 
             <h2><a name="demons">Demonstrate</a></h2>
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             <p>Using this project as a foundation other groups should be able to take a step further with their quorum sensing projects' design, by having the possibility of quantifying more precisely the interaction between different systems, and use it to improve their modelling. Furthermore, we took into account the time needed to produce the components of the system, generating a more realistic result. The next step must be to consider the cross talk between systems that was measured and work to minimize them, creating more orthogonal circuits.  
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             <p>Using this project as a foundation other groups should be able to take a step further with their quorum sensing projects' design, by having the possibility of quantifying more precisely the interaction between different systems, and use it to improve their modelling. Furthermore, we took into account the time needed to produce the components of the system, generating a more realistic result. The next step must be to consider the cross talk between systems that was measured and work to minimize them, creating increasingly orthogonal circuits.  
 
             </p>
 
             </p>
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            <p>We have been successful in our initial proposition, of making a modular construct thas was capable of measuring quorum sensing interactions. Our Lux Sender system (<a href="http://parts.igem.org/Part:BBa_K2771030">BBa_K2771030</a>) worked as expected, generating a Homoserine Lactone signal capable of activating a collection of quorum sensing promoters. We also established a reliable method for measuring this activity, with a ratiometric activity reporter that gives us an adimensional, robust measurement, by utilizing EYFP as a reporter and CFP as normalization control.
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            </p>
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            <div class="column half_size">
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              <center><img src="https://static.igem.org/mediawiki/2018/5/5f/T--USP-Brazil--Design_Scheme.png" style="width:90%"></center>
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              <h6>Proposed system</h6>
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            </div>
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            <div class="column half_size">
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              <center><img src="https://static.igem.org/mediawiki/2018/3/38/T--USP-Brazil--Lux_Lux_petri_dish.png" style="width:90%"></center>
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              <h6>Correct expected Result</h6>
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            </div>
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              <p>With this system in hands
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              </p>
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              <p>
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              </p>
 
           </div>
 
           </div>
 
         </article>     
 
         </article>     

Revision as of 21:46, 17 October 2018

Wiki - iGEM Brazil

Demonstrate

Using this project as a foundation other groups should be able to take a step further with their quorum sensing projects' design, by having the possibility of quantifying more precisely the interaction between different systems, and use it to improve their modelling. Furthermore, we took into account the time needed to produce the components of the system, generating a more realistic result. The next step must be to consider the cross talk between systems that was measured and work to minimize them, creating increasingly orthogonal circuits.

We have been successful in our initial proposition, of making a modular construct thas was capable of measuring quorum sensing interactions. Our Lux Sender system (BBa_K2771030) worked as expected, generating a Homoserine Lactone signal capable of activating a collection of quorum sensing promoters. We also established a reliable method for measuring this activity, with a ratiometric activity reporter that gives us an adimensional, robust measurement, by utilizing EYFP as a reporter and CFP as normalization control.

Proposed system
Correct expected Result

With this system in hands