Difference between revisions of "Team:Austin LASA"
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| − | <p> | + | <p>HIV-1 is a retrovirus that ultimately leads to AIDS and can be passed from a mother to a child during pregnancy, delivery, or breastfeeding. Early intervention of HIV-1 reduces infant mortality by up to 75%, but currently samples often have to be sent off to labs with more resources than are present in the immediate area. Easier detection of HIV through a Cas12a-based system will aid in earlier detection, as assays based on antibody detection and PCR are inaccurate in infants and difficult to administer in the field, respectively. The end goal of our project is to make a transportable kit that would hypothetically be able to be used in the field for the detection of HIV-1 in infants. We will be doing so by using cellular reagents, which takes the sequences for important reactants and inserts them into an E. coli cell. We fill first be amplifying the viral DNA using LAMP and then detecting it using a CRISPR-cas12a system paired with a fluorophore-quencher assay. Ultimately, we wish to produce a "kit" that could be used in the field for rapid and simple detection of HIV-1.</p> |
Revision as of 18:32, 2 July 2018
Welcome to iGEM 2018!
HIV-1 is a retrovirus that ultimately leads to AIDS and can be passed from a mother to a child during pregnancy, delivery, or breastfeeding. Early intervention of HIV-1 reduces infant mortality by up to 75%, but currently samples often have to be sent off to labs with more resources than are present in the immediate area. Easier detection of HIV through a Cas12a-based system will aid in earlier detection, as assays based on antibody detection and PCR are inaccurate in infants and difficult to administer in the field, respectively. The end goal of our project is to make a transportable kit that would hypothetically be able to be used in the field for the detection of HIV-1 in infants. We will be doing so by using cellular reagents, which takes the sequences for important reactants and inserts them into an E. coli cell. We fill first be amplifying the viral DNA using LAMP and then detecting it using a CRISPR-cas12a system paired with a fluorophore-quencher assay. Ultimately, we wish to produce a "kit" that could be used in the field for rapid and simple detection of HIV-1.
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples: