Difference between revisions of "Team:ZJU-China/AllParts"

Revision as of 23:55, 17 October 2018

<!DOCTYPE html> Electrode

ALL PARTS

OVERVIEW

This year, we designed 23 parts,and all of them had been submitted to the iGEM Registry following the BioBricks assembly standard as described by RFC10.

Here is a brief description of our parts. If you want to get more detailed information about our parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.

Parts Table

Name	Type	Description	Length(bp)	Designer
BBa_K2721000	promoters	Modified T7 promoter 1	23	Huining Liang
BBa_K2721001	promoters	Modified T7 promoter 2	23	Huining Liang
BBa_K2721002	promoters	Modified T7 promoter 3	23	Huining Liang
BBa_K2721003	promoters	Modified T7 promoter 4	23	Huining Liang
BBa_K2721000	coding	Gene of csgA	525	Xinhui Li
BBa_K2721001	composite part	modified csgA expression system	893	Xinhui Li
BBa_K2721020	coding	Spycatcher with histag	306	Yunqian Li
BBa_K2721021	coding	Snoopcatcher with histag	366	Yunqian Li
BBa_K2721022	coding	Spycatcher with histag	369	Yunqian Li
BBa_K2721023	coding	SpyTag with GFP	759	Yunqian Li
BBa_K2721024	coding	RFP with SdyTag and SpyCatcher	1047	Yunqian Li
BBa_K2721025	coding	GFP with SnoopTag and SdyCatcher	1131	Yunqian Li
BBa_K2721026	coding	Snoopcatcher with YFP	1092	Yunqian Li
BBa_K2721027	coding	LDH with SdyTag and SpyCatcher	1116	Yunqian Li
BBa_K2721028	coding	Glucose Oxidase(GOX) with SnoopCatcher	2157	Yunqian Li
BBa_K2721029	coding	HRP with SnoopTag and SdyCatcher	1374	Yunqian Li
BBa_K2721030	composite part	SpyTag with GFP	1124	Yunqian Li
BBa_K2721031	composite part	RFP with SdyTag and SpyCatcher	943	Yunqian Li
BBa_K2721032	composite part	GFP with SnoopTag and SdyCatcher	1315	Yunqian Li
BBa_K2721033	composite part	Snoopcatcher with YFP	1274	Yunqian Li
BBa_K2721034	composite part	LDH with SdyTag and SpyCatcher	1300	Yunqian Li
BBa_K2721035	composite part	Glucose Oxidase(GOX) with SnoopCatcher	2341	Yunqian Li
BBa_K2721036	composite part	HRP with SnoopTag and SdyCatcher	1558	Yunqian Li

Best New Basic Part

BBa_k2721010

Curli is the major component of extra-cellular matrix produced by certain strains of Enterobacteriaceae. This part codes for CsgA, the monomer of Curli fibers which is an ideal material of biofilm. Click here to see more details.

Best New Composite Part

BBa_k2721036

This part codes for HRP(Horseradish Peroxidase), SnoopTag and SdyCatcehr. Target DNA sequence of this part was optimized and synthesized. We cloned the synthesized sequence into vector PET-27(+) and successfully detected the production. Click here to see more details.

Best Part Collection

BBa_k2721027~BBa_k2721029

The main biofunction of our final product lies in the three connected enzymes. So the best part collection codes for the three enzymes LDH(Lactate Dehydrogenase), GOX (Glucose Oxidase) and HRP(Horseradish Peroxidase) separately flanked by different tag-catcher pairs. Click here to see more details.

Improve T7 promoters

BBa_k2721000~BBa_k2721003

Known for its highly yields of recombinant proteins, T7 promoter is currently one of the most widely used promoters in genetic engineering. This year, we did Site-directed mutagenesis to get some mutants with even higher efficiency of protein expression. We chose GFP as a reporter to test the function of our modified T7 promoters. Click here to see more details.

Our Sponsors

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 								<a href="#" class="dropdown-toggle" data-toggle="dropdown">Applied Design</a>
 								<ul class="dropdown-menu">
-									<li><a href="https://2018.igem.org/Team:ZJU-China/ProductDesign">Product Design</a></li>
+									<li><a href="https://2018.igem.org/Team:ZJU-China/Applied_Design">Product Design</a></li>
 									<li><a href="https://2018.igem.org/Team:ZJU-China/Hardware">Hardware</a></li>