Difference between revisions of "Team:Munich/assembly1.html"

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<h4>T7, T4, P2, P3 Phage Assembly in Commercial Cell-Extract </h4>
 
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<em>2018/07/04- 2018/04/05</em>
 
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<table class="table table-borderless">
 
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    <tr>
 
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      <td>Participants:</td>
 
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      <td>Brigit Tunaj</td>
 
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    </tr>
 
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    <tr>
 
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      <td>Protocol:</td>
 
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      <td>
 
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<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">PhageT7AssemblyTXTL</a>,
 
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<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a>
 
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</td>
 
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    </tr>
 
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    <tr>
 
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      <td>Notes:</td>
 
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      <td> No notes
 
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    </tr>
 
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<tr>
 
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      <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
 
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      <td> T7 Phage:  6 *10^9 PFU/mL
 
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</td>
 
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    </tr>
 
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</table>
 
 
   
 
   
 
<h4>T7 Phage Titer Experiment with TEM </h4>
 
<h4>T7 Phage Titer Experiment with TEM </h4>

Revision as of 02:27, 18 October 2018

T7 Phage Assembly in Commercial Cell-Extract

2018/05/17- 2018/05/18
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: No notes
Results: No results

MS2 Phage Assembly in Commercial Cell-Extract

2018/05/17- 2018/05/18
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: No notes
Results: No results

T7 Phage Assembly in Self-Produced Cell-Extract

2018/06/04- 2018/06/05
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 80 µL batch
Results: No results

T7 and MS2 Phage Assembly in Self-Produced Cell-Extract

2018/06/05- 2018/06/06
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 25 µL batch
Results: No results

T7 and MS2 Phage Assembly in Self-Produced Cell-Extract

2018/06/06- 2018/06/07
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 20 µL batch
Results: No results

T7 Phage Assembly in Self-Produced Cell-Extract

2018/06/07- 2018/06/08
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 40 µL batch and different DNA (55 nM)
Results: No results

T7 Phage Assembly in Self-Produced Cell-Extract

2018/06/11- 2018/06/12
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 40 µL batch and various of DNA-water relation (à more DNA)
Results: No results

T7 Phage Assembly in Self-Produced Cell-Extract

2018/06/13- 2018/06/14
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 40 µL batch and various of DNA-water relation (à more DNA)
Results: No results

T7 Phage Assembly in Self-Produced Cell-Extract

2018/06/14- 2018/06/15
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 25 µL batch, DNA (10,37 nM)
Results: No results

T7 Phage Assembly in Commercial Cell-Extract

2018/06/18- 2018/06/19
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: 25 µl batch, DNA (10,37 nM)
Results: T7 Phage: 3 *10^5 PFU/mL

T7 Phage Assembly in Commercial Cell-Extract

2018/06/21- 2018/06/22
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: DNA (10,37 nM)
Results: No results

T7 Phage Assembly in Commercial Cell-Extract

2018/06/26- 2018/06/27
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: DNA (10,37 nM), reaction started in the evening for just 7 hours
Results: T7 Phage: 1,5*10^7 PFU/mL

T7 Phage Assembly in Commercial Cell-Extract

2018/06/26- 2018/06/27
Participants: Brigit Tunaj
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: DNA (10,37 nM), reaction started in the evening for just 7 hours
Results: T7 Phage: 3 *10^7 PFU/mL

T7 Phage Titer Experiment with TEM

2018/06/07- 2018/05/18
Participants: Brigit Tunaj, Kilian Vogele
Protocol: TEM preparation,
Notes: No notes
Results: Picture