Difference between revisions of "Team:UofGuelph/Contributions"

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<h1 class="descSub">2018 Registry Submissions</h1>
 
<h1 class="descSub">2018 Registry Submissions</h1>
 
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Add descriptions here
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Sequencing of BBa_K747047
 
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<h1 class="descSub">Background</h1>
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<p class="descP">
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iGEM Guelph's 2018 contribution to the registry was the sequence analysis of a BioBrick located in the 2018 Distribution Kit.<br><br>
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BioBrick K747047 was located in Plate 2, Well 12O. Our team chose to sequence this part as the Distribution Kit listed it as a Bad Sequence and for our team’s first characterization of a Registry Part, we thought that this part was a good candidate. Sequencing preparation was performed by our team, while the sequencing was performed at the Laboratory Services at the University of Guelph.
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</p>
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<h1 class="descSub">Methods</h1>
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<p class="descP">
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A PCR was set up according to the following recipe:
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<table style="width:50%">
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  <tr>
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    <th>Component</th>
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    <th>Volume (µL)</th>
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  </tr>
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  <tr>
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    <td>H<sub>2</sub>O</td>
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    <td>20.4</td>
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  </tr>
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    <td>Phusion Buffer</td>
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    <td>8.0</td>
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  </tr>
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    <td>dNTPs</td>
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    <td>0.8</td>
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  </tr>
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    <td>VF2</td>
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    <td>0.2</td>
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  </tr>
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  <tr>
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    <td>VR</td>
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    <td>0.2</td>
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  </tr>
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  <tr>
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    <td>BBa_DNA</td>
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    <td>10</td>
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  </tr>
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  <tr>
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    <td>Phusion</td>
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    <td>0.4</td>
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  </tr>
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  <tr>
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    <td><b>Total</b></td>
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    <td><b>40</b></td>
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  </tr>
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</table>
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Revision as of 03:06, 18 October 2018

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Registry Contributions

2018 Registry Submissions

Sequencing of BBa_K747047

Background

iGEM Guelph's 2018 contribution to the registry was the sequence analysis of a BioBrick located in the 2018 Distribution Kit.

BioBrick K747047 was located in Plate 2, Well 12O. Our team chose to sequence this part as the Distribution Kit listed it as a Bad Sequence and for our team’s first characterization of a Registry Part, we thought that this part was a good candidate. Sequencing preparation was performed by our team, while the sequencing was performed at the Laboratory Services at the University of Guelph.

Methods

A PCR was set up according to the following recipe:

Component Volume (µL)
H2O 20.4
Phusion Buffer 8.0
dNTPs 0.8
VF2 0.2
VR 0.2
BBa_DNA 10
Phusion 0.4
Total 40

University of Guelph iGEM 2017