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| − | <!-- i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way !--> | + | <div class="columnLeft c66"> <!-- Where the actual content actually is !--> |
| − |
| + | <div class=" meatyRegion"> |
| − | <div class=" meatSection"> | + | |
| − | | + | |
| − | | + | Synthetic biology is all about collaboration between scientists in an attempt to further our understanding of the field and improve the world around us. However, with the variability that occurs in synthetic biology measurement, it is much harder to consistently build on work done by others. This year, the 5th Interlab Study was designed to reduce lab to lab variability in fluorescence by normalizing data to Colony Forming Units (CFU). The 2018 UMaryland iGEM team has performed the interlab study using the <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf"> <u>protocol</ u></ a> distributed by the Measurement Committee. |
| − | <p>We did this year's iGEM InterLab study. It was tremendous.</p>
| + | <br><br> |
| − | <p>We followed the protocol given by iGEM absolutely perfectly. Look at the beautiful protocol we followed <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf"> here</ a> .</ p> | + | Feel free to download our data <a href="https://static.igem.org/mediawiki/2018/e/ec/T--UMaryland--interlab_excel_sheet.xlsx" ><u>here </u></a>. |
| − | < p> Download our excel file we actually used <a href="https://static.igem.org/mediawiki/2018/e/ec/T--UMaryland--interlab_excel_sheet.xlsx">here</a>.</ p> | + | </div> |
| | </div> | | </div> |
| − | <div id="Calibration"> | + | <div id="procedures"> |
| − | <h2>Calibration</h2> | + | <div class="meatSubtitle"> |
| − | <h3>OD600 Reference Point - LUDOX Protocol</h3>
| + | Procedures |
| − | <table id="calibrationTable">
| + | </div> |
| − | <tr>
| + | <div class="meatMeat"> |
| − | <th></th>
| + | All measurements were performed using a standard clear 96-well plate. Calibration of our microplate reader used LUDOX CL-X as a single point reference to appropriately convert absorbance values into a standard OD600 measurement. Absorbances were measured for four replicates per LUDOX and deionized water at 600 nm. The particle standard curve was produced using silica beads and measured at 600 nm. There was also a fluorescence standard curve produced by using fluorescein and 1xPBS solution. Once all calibrations were completed, transformations of all devices in DH5-α, including positive and negative controls took place. Two colonies were chosen from each and incubated in 5 mL of LB media with chloramphenicol. They were left overnight for 16 hours at 37 C at around 220 rpm. All samples were immediately placed on ice and 100 ul was pipetted into the 96 well plate at the 0 and 6h timepoints. The absorbance and fluorescence was measured for both. The CFU measurements were taken by diluting the overnight cultures to an OD of 0.1. The samples were then diluted 1:20 three times and then 1:10 another two times. The final three dilutions for each replicate were plated and colonies were counted after 18 hours of growth at 37 C to determine the CFU/ml as can be seen in our results section. |
| − | <th>LUDOX CL-X</th>
| + | </div> |
| − | <th>H2O</th>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Replicate 1</th>
| + | |
| − | <td class="blueCells">0.058</td>
| + | |
| − | <td class="blueCells">0.034</td>
| + | |
| − | </tr> | + | |
| − | <tr>
| + | |
| − | <th>Replicate 2</th>
| + | |
| − | <td class="blueCells">0.056</td>
| + | |
| − | <td class="blueCells">0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Replicate 3</th>
| + | |
| − | <td class="blueCells">0.058</td>
| + | |
| − | <td class="blueCells">0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Replicate 4</th>
| + | |
| − | <td class="blueCells">0.058</td>
| + | |
| − | <td class="blueCells">0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Arithmetic Mean</th>
| + | |
| − | <td class="yellowCells">0.058</td>
| + | |
| − | <td class="yellowCells">0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Corrected Abs600</th>
| + | |
| − | <td class="yellowCells">0.058</td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Reference OD600</th>
| + | |
| − | <td class="yellowCells">0.058</td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>OD600/Abs600</th>
| + | |
| − | <td class="yellowCells">0.058</td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table> | + | |
| − | <h3>Particle Standard Curve - Microsphere Protocol</h3> | + | |
| − | <table id="microsphereTable">
| + | |
| − | <tr>
| + | |
| − | <th>Number of Particles</th>
| + | |
| − | <th>2.35×10<sup>8</sup></th>
| + | |
| − | <th>1.18×10<sup>8</sup></th>
| + | |
| − | <th>5.88×10<sup>7</sup></th>
| + | |
| − | <th>2.94×10<sup>7</sup></th>
| + | |
| − | <th>1.47×10<sup>7</sup></th>
| + | |
| − | <th>7.35×10<sup>6</sup></th>
| + | |
| − | <th>3.68×10<sup>6</sup></th>
| + | |
| − | <th>1.84×10<sup>6</sup></th>
| + | |
| − | <th>9.19×10<sup>5</sup></th>
| + | |
| − | <th>4.60×10<sup>5</sup></th>
| + | |
| − | <th>2.30×10<sup>5</sup></th>
| + | |
| − | <th>0</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 1</th>
| + | |
| − | <td>0.716</td>
| + | |
| − | <td>0.322</td>
| + | |
| − | <td>0.191</td>
| + | |
| − | <td>0.095</td>
| + | |
| − | <td>0.067</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | <td>0.039</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.034</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 2</th>
| + | |
| − | <td>0.406</td>
| + | |
| − | <td>0.359</td>
| + | |
| − | <td>0.212</td>
| + | |
| − | <td>0.111</td>
| + | |
| − | <td>0.072</td>
| + | |
| − | <td>0.051</td>
| + | |
| − | <td>0.044</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.034</td>
| + | |
| − | <td>0.034</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 3</th>
| + | |
| − | <td>0.587</td>
| + | |
| − | <td>0.347</td>
| + | |
| − | <td>0.203</td>
| + | |
| − | <td>0.116</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>-</td>
| + | |
| − | <td>0.041</td>
| + | |
| − | <td>0.038</td>
| + | |
| − | <td>0.038</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.034</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 4</th>
| + | |
| − | <td>0.815</td>
| + | |
| − | <td>0.419</td>
| + | |
| − | <td>0.219</td>
| + | |
| − | <td>0.123</td>
| + | |
| − | <td>0.081</td>
| + | |
| − | <td>0.051</td>
| + | |
| − | <td>0.048</td>
| + | |
| − | <td>0.040</td>
| + | |
| − | <td>0.038</td>
| + | |
| − | <td>0.037</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Mean</th>
| + | |
| − | <td>0.631</td>
| + | |
| − | <td>0.364</td>
| + | |
| − | <td>0.206</td>
| + | |
| − | <td>0.111</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.054</td>
| + | |
| − | <td>0.043</td>
| + | |
| − | <td>0.038</td>
| + | |
| − | <td>0.037</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Standard Deviation</th>
| + | |
| − | <td>0.177</td>
| + | |
| − | <td>0.038</td>
| + | |
| − | <td>0.012</td>
| + | |
| − | <td>0.012</td>
| + | |
| − | <td>0.006</td>
| + | |
| − | <td>0.005</td>
| + | |
| − | <td>0.004</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.001</td>
| + | |
| − | <td>0.001</td>
| + | |
| − | <td>0.001</td>
| + | |
| − | <td>0.001</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Net Mean</th>
| + | |
| − | <td>0.596</td>
| + | |
| − | <td>0.329</td>
| + | |
| − | <td>0.172</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.039</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.008</td>
| + | |
| − | <td>0.003</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.001</td>
| + | |
| − | <td>0.000</td>
| + | |
| − | <td class="whiteCellsImportant"></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/b/bb/T--UMaryland--interlab_particle_standard_curve.png" alt="interlab particle standard curve">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/7/77/T--UMaryland--interlab_particle_standard_curve_log.png" alt="interlab particle standard curve">
| + | |
| − | <table id="particlesPerOD">
| + | |
| − | <tr>
| + | |
| − | <th>Number of Particles</th>
| + | |
| − | <th>2.35×10<sup>8</sup></th>
| + | |
| − | <th>1.18×10<sup>8</sup></th>
| + | |
| − | <th>5.88×10<sup>7</sup></th>
| + | |
| − | <th>2.94×10<sup>7</sup></th>
| + | |
| − | <th>1.47×10<sup>7</sup></th>
| + | |
| − | <th>7.35×10<sup>6</sup></th>
| + | |
| − | <th>3.68×10<sup>6</sup></th>
| + | |
| − | <th>1.84×10<sup>6</sup></th>
| + | |
| − | <th>9.19×10<sup>5</sup></th>
| + | |
| − | <th>4.60×10<sup>5</sup></th>
| + | |
| − | <th>2.30×10<sup>5</sup></th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">µM Fluorescein/a.u.</th>
| + | |
| − | <td>3.95×10<sup>8</sup></td>
| + | |
| − | <td>3.57×10<sup>8</sup></td>
| + | |
| − | <td>3.43×10<sup>8</sup></td>
| + | |
| − | <td>3.84×10<sup>8</sup></td>
| + | |
| − | <td>3.77×10<sup>8</sup></td>
| + | |
| − | <td>3.89×10<sup>8</sup></td>
| + | |
| − | <td>4.46×10<sup>8</sup></td>
| + | |
| − | <td>6.68×10<sup>8</sup></td>
| + | |
| − | <td>4.80×10<sup>8</sup></td>
| + | |
| − | <td>4.60×10<sup>8</sup></td>
| + | |
| − | <td>-</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>MEFL/a.u.</th>
| + | |
| − | <td></td>
| + | |
| − | <td class="yellowCells">3.70×10<sup>8</sup></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h3>Fluorescence Standard Curve - Fluorescein Protocol</h3>
| + | |
| − | <table id="fluoresceinTable">
| + | |
| − | <tr>
| + | |
| − | <th>Fluorescein µM</th>
| + | |
| − | <th>10</th>
| + | |
| − | <th>5</th>
| + | |
| − | <th>2.5</th>
| + | |
| − | <th>1.25</th>
| + | |
| − | <th>0.625</th>
| + | |
| − | <th>0.313</th>
| + | |
| − | <th>0.156</th>
| + | |
| − | <th>0.078</th>
| + | |
| − | <th>0.039</th>
| + | |
| − | <th>0.0195</th>
| + | |
| − | <th>0.0098</th>
| + | |
| − | <th>0</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 1</th>
| + | |
| − | <td>27845</td>
| + | |
| − | <td>21611</td>
| + | |
| − | <td>15480</td>
| + | |
| − | <td>10321</td>
| + | |
| − | <td>5979</td>
| + | |
| − | <td>3376</td>
| + | |
| − | <td>1806</td>
| + | |
| − | <td>891</td>
| + | |
| − | <td>442</td>
| + | |
| − | <td>244</td>
| + | |
| − | <td>122</td>
| + | |
| − | <td>2</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 2</th>
| + | |
| − | <td>27711</td>
| + | |
| − | <td>19390</td>
| + | |
| − | <td>16110</td>
| + | |
| − | <td>10742</td>
| + | |
| − | <td>6223</td>
| + | |
| − | <td>3240</td>
| + | |
| − | <td>1756</td>
| + | |
| − | <td>895</td>
| + | |
| − | <td>467</td>
| + | |
| − | <td>227</td>
| + | |
| − | <td>115</td>
| + | |
| − | <td>2</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 3</th>
| + | |
| − | <td>27686</td>
| + | |
| − | <td>21709</td>
| + | |
| − | <td>15590</td>
| + | |
| − | <td>9458</td>
| + | |
| − | <td>5304</td>
| + | |
| − | <td>2972</td>
| + | |
| − | <td>1350</td>
| + | |
| − | <td>711</td>
| + | |
| − | <td>481</td>
| + | |
| − | <td>101</td>
| + | |
| − | <td>160</td>
| + | |
| − | <td>3</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Replicate 4</th>
| + | |
| − | <td>27431</td>
| + | |
| − | <td>21442</td>
| + | |
| − | <td>15379</td>
| + | |
| − | <td>9474</td>
| + | |
| − | <td>5366</td>
| + | |
| − | <td>2703</td>
| + | |
| − | <td>1427</td>
| + | |
| − | <td>716</td>
| + | |
| − | <td>359</td>
| + | |
| − | <td>197</td>
| + | |
| − | <td>115</td>
| + | |
| − | <td>3</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Mean</th>
| + | |
| − | <td>27668</td>
| + | |
| − | <td>21038</td>
| + | |
| − | <td>15640</td>
| + | |
| − | <td>10000</td>
| + | |
| − | <td>5718</td>
| + | |
| − | <td>3073</td>
| + | |
| − | <td>1585</td>
| + | |
| − | <td>803</td>
| + | |
| − | <td>437</td>
| + | |
| − | <td>192</td>
| + | |
| − | <td>128</td>
| + | |
| − | <td>2.5</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Standard Deviation</th>
| + | |
| − | <td>172.8</td>
| + | |
| − | <td>1104</td>
| + | |
| − | <td>325.1</td>
| + | |
| − | <td>638.8</td>
| + | |
| − | <td>454.0</td>
| + | |
| − | <td>298.2</td>
| + | |
| − | <td>229.7</td>
| + | |
| − | <td>103.7</td>
| + | |
| − | <td>54.60</td>
| + | |
| − | <td>63.86</td>
| + | |
| − | <td>21.59</td>
| + | |
| − | <td>0.5774</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Arithmetic Net Mean</th>
| + | |
| − | <td>27666</td>
| + | |
| − | <td>21036</td>
| + | |
| − | <td>15637</td>
| + | |
| − | <td>9996</td>
| + | |
| − | <td>5716</td>
| + | |
| − | <td>3070</td>
| + | |
| − | <td>1582</td>
| + | |
| − | <td>801</td>
| + | |
| − | <td>435</td>
| + | |
| − | <td>190</td>
| + | |
| − | <td>126</td>
| + | |
| − | <td class="whiteCellsImportant"></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/2/29/T--UMaryland--interlab_flourescein_standard_curve.png" alt="interlab particle standard curve">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/0/02/T--UMaryland--interlab_flourescein_standard_curve_log.png" alt="interlab particle standard curve">
| + | |
| − | <table id="flouresceinAU">
| + | |
| − | <tr>
| + | |
| − | <th>Flourescein µM</th>
| + | |
| − | <th>10.00</th>
| + | |
| − | <th>5.00</th>
| + | |
| − | <th>2.50</th>
| + | |
| − | <th>1.25</th>
| + | |
| − | <th>0.63</th>
| + | |
| − | <th>0.31</th>
| + | |
| − | <th>0.16</th>
| + | |
| − | <th>0.08</th>
| + | |
| − | <th>0.04</th>
| + | |
| − | <th>0.02</th>
| + | |
| − | <th>0.01</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">µM Fluorescein/a.u.</th>
| + | |
| − | <td>3.61×10<sup>-4</sup></td>
| + | |
| − | <td>2.38×10<sup>-4</sup></td>
| + | |
| − | <td>1.60×10<sup>-4</sup></td>
| + | |
| − | <td>1.25×10<sup>-4</sup></td>
| + | |
| − | <td>1.09×10<sup>-4</sup></td>
| + | |
| − | <td>1.02×10<sup>-4</sup></td>
| + | |
| − | <td>9.88×10<sup>-5</sup></td>
| + | |
| − | <td>9.76×10<sup>-5</sup></td>
| + | |
| − | <td>8.99×10<sup>-5</sup></td>
| + | |
| − | <td>1.03×10<sup>-4</sup></td>
| + | |
| − | <td>7.78×10<sup>-5</sup></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>Mean µM Fluorescein/a.u.</th>
| + | |
| − | <td></td>
| + | |
| − | <td class="yellowCells">1.47×10<sup>-4</sup></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <th>MEFL/a.u.</th>
| + | |
| − | <td></td>
| + | |
| − | <td class="yellowCells">8.84×10<sup>8</sup></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table> | + | |
| | </div> | | </div> |
| − | <div id="Cell Measurement"> | + | <div id="calibration"> |
| − | <h2>Cell Measurement</h2>
| + | <div class="meatSubtitle"> |
| − | <div id="Raw Plate Reader Measurements"> | + | 1. Calibration |
| − | <h3>Plate Reader Raw Measurement</h3> | + | </div> |
| − | <h4>Fluorescence Raw Readings</h4>
| + | <div class="meatMeat"> |
| − | <h5>Hour 0:</h5>
| + | OD600 Reference Point - LUDOX Protocol |
| − | <table id="FlourescenceRawReadingsHour0">
| + | <br> |
| − | <tr>
| + | <div class="center"> |
| − | <th></th>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab1.png" alt="waluigi time"> |
| − | <th>Negative Control</th>
| + | </div> |
| − | <th>Positive Control</th>
| + | </div> |
| − | <th>Device 1</th>
| + | <div class="meatMeat"> |
| − | <th>Device 2</th>
| + | Particle Standard Curve - Microsphere Protocol |
| − | <th>Device 3</th>
| + | <br> |
| − | <th>Device 4</th>
| + | <div class="center"> |
| − | <th>Device 5</th>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab2.png" alt="waluigi time"> |
| − | <th>Device 6</th>
| + | <img src="https://static.igem.org/mediawiki/2018/b/bb/T--UMaryland--interlab_particle_standard_curve.png" alt="interlab particle standard curve"> |
| − | <th>LB + Chloro. (blank)</th>
| + | <img src="https://static.igem.org/mediawiki/2018/7/77/T--UMaryland--interlab_particle_standard_curve_log.png" alt="interlab particle standard curve"> |
| − | </tr>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab3.png" alt="waluigi time"> |
| − | <tr class="blueCells">
| + | </div> |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 1</th>
| + | </div> |
| − | <td>23</td>
| + | <div class="meatMeat"> |
| − | <td>43</td>
| + | Fluorescence Standard Curve - Fluorescein Protocol |
| − | <td>63</td>
| + | <br> |
| − | <td>44</td>
| + | <div class="center"> |
| − | <td>24</td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab4.png" alt="waluigi time"> |
| − | <td>84</td>
| + | <img src="https://static.igem.org/mediawiki/2018/2/29/T--UMaryland--interlab_flourescein_standard_curve.png" alt="interlab particle standard curve"> |
| − | <td>63</td>
| + | <img src="https://static.igem.org/mediawiki/2018/0/02/T--UMaryland--interlab_flourescein_standard_curve_log.png" alt="interlab particle standard curve"> |
| − | <td>55</td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab5.png" alt="waluigi time"> |
| − | <td>23</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells"> | + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 2</th>
| + | |
| − | <td>25</td>
| + | |
| − | <td>36</td>
| + | |
| − | <td>51</td>
| + | |
| − | <td>36</td>
| + | |
| − | <td>21</td>
| + | |
| − | <td>69</td>
| + | |
| − | <td>52</td>
| + | |
| − | <td>44</td>
| + | |
| − | <td>20</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 3</th> | + | |
| − | <td>25</td>
| + | |
| − | <td>35</td>
| + | |
| − | <td>51</td>
| + | |
| − | <td>38</td>
| + | |
| − | <td>23</td>
| + | |
| − | <td>70</td>
| + | |
| − | <td>52</td>
| + | |
| − | <td>44</td>
| + | |
| − | <td>20</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 4</th>
| + | |
| − | <td>19</td>
| + | |
| − | <td>37</td>
| + | |
| − | <td>54</td>
| + | |
| − | <td>35</td>
| + | |
| − | <td>23</td>
| + | |
| − | <td>70</td>
| + | |
| − | <td>53</td>
| + | |
| − | <td>48</td>
| + | |
| − | <td>21</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 1</th>
| + | |
| − | <td>25</td>
| + | |
| − | <td>35</td>
| + | |
| − | <td>80</td>
| + | |
| − | <td>29</td>
| + | |
| − | <td>25</td>
| + | |
| − | <td>74</td>
| + | |
| − | <td>68</td>
| + | |
| − | <td>38</td>
| + | |
| − | <td>21</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 2</th>
| + | |
| − | <td>28</td>
| + | |
| − | <td>34</td>
| + | |
| − | <td>81</td>
| + | |
| − | <td>31</td>
| + | |
| − | <td>25</td>
| + | |
| − | <td>75</td>
| + | |
| − | <td>69</td>
| + | |
| − | <td>39</td>
| + | |
| − | <td>22</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 3</th>
| + | |
| − | <td>27</td>
| + | |
| − | <td>35</td>
| + | |
| − | <td>83</td>
| + | |
| − | <td>32</td>
| + | |
| − | <td>25</td>
| + | |
| − | <td>75</td>
| + | |
| − | <td>68</td>
| + | |
| − | <td>40</td>
| + | |
| − | <td>21</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 4</th>
| + | |
| − | <td>31</td>
| + | |
| − | <td>46</td>
| + | |
| − | <td>105</td>
| + | |
| − | <td>40</td>
| + | |
| − | <td>34</td>
| + | |
| − | <td>101</td>
| + | |
| − | <td>90</td>
| + | |
| − | <td>52</td>
| + | |
| − | <td>27</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h5>Hour 6:</h5>
| + | |
| − | <table id="FlourescenceRawReadingsHour6">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | <th>LB + Chloro. (blank)</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 1</th>
| + | |
| − | <td>31</td>
| + | |
| − | <td>288</td>
| + | |
| − | <td>447</td>
| + | |
| − | <td>251</td>
| + | |
| − | <td>37</td>
| + | |
| − | <td>132</td>
| + | |
| − | <td>111</td>
| + | |
| − | <td>205</td>
| + | |
| − | <td>24</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 2</th>
| + | |
| − | <td>36</td>
| + | |
| − | <td>266</td>
| + | |
| − | <td>382</td>
| + | |
| − | <td>213</td>
| + | |
| − | <td>36</td>
| + | |
| − | <td>112</td>
| + | |
| − | <td>93</td>
| + | |
| − | <td>186</td>
| + | |
| − | <td>20</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 3</th>
| + | |
| − | <td>35</td>
| + | |
| − | <td>252</td>
| + | |
| − | <td>382</td>
| + | |
| − | <td>216</td>
| + | |
| − | <td>37</td>
| + | |
| − | <td>108</td>
| + | |
| − | <td>102</td>
| + | |
| − | <td>178</td>
| + | |
| − | <td>21</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells"> | + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 4</th>
| + | |
| − | <td>36</td>
| + | |
| − | <td>260</td>
| + | |
| − | <td>381</td>
| + | |
| − | <td>216</td>
| + | |
| − | <td>39</td>
| + | |
| − | <td>113</td>
| + | |
| − | <td>93</td>
| + | |
| − | <td>179</td>
| + | |
| − | <td>22</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 1</th> | + | |
| − | <td>36</td>
| + | |
| − | <td>260</td>
| + | |
| − | <td>381</td>
| + | |
| − | <td>216</td>
| + | |
| − | <td>39</td>
| + | |
| − | <td>116</td>
| + | |
| − | <td>140</td>
| + | |
| − | <td>161</td>
| + | |
| − | <td>24</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 2</th>
| + | |
| − | <td>39</td>
| + | |
| − | <td>215</td>
| + | |
| − | <td>340</td>
| + | |
| − | <td>152</td>
| + | |
| − | <td>110</td>
| + | |
| − | <td>121</td>
| + | |
| − | <td>137</td>
| + | |
| − | <td>163</td>
| + | |
| − | <td>24</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 3</th>
| + | |
| − | <td>37</td>
| + | |
| − | <td>210</td>
| + | |
| − | <td>348</td>
| + | |
| − | <td>150</td>
| + | |
| − | <td>108</td>
| + | |
| − | <td>117</td>
| + | |
| − | <td>139</td>
| + | |
| − | <td>161</td>
| + | |
| − | <td>23</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 4</th>
| + | |
| − | <td>41</td>
| + | |
| − | <td>258</td>
| + | |
| − | <td>415</td>
| + | |
| − | <td>175</td>
| + | |
| − | <td>130</td>
| + | |
| − | <td>150</td>
| + | |
| − | <td>183</td>
| + | |
| − | <td>198</td>
| + | |
| − | <td>23</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h4>Abs600 Raw Readings</h4>
| + | |
| − | <h5>Hour 0:</h5>
| + | |
| − | <table id="Abs600RawReadingsHour0">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | <th>LB + Chloro. (blank)</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 1</th>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.073</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.078</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.068</td>
| + | |
| − | <td>0.068</td>
| + | |
| − | <td>0.068</td>
| + | |
| − | <td>0.060</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 2</th>
| + | |
| − | <td>0.079</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.069</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.054</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 3</th>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.062</td>
| + | |
| − | <td>0.078</td>
| + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 4</th>
| + | |
| − | <td>0.068</td>
| + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.080</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.071</td>
| + | |
| − | <td>0.073</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.057</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 1</th>
| + | |
| − | <td>0.081</td> | + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.084</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.072</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.079</td>
| + | |
| − | <td>0.078</td>
| + | |
| − | <td>0.060</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 2</th>
| + | |
| − | <td>0.085</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.083</td>
| + | |
| − | <td>0.079</td>
| + | |
| − | <td>0.072</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.082</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 3</th>
| + | |
| − | <td>0.081</td> | + | |
| − | <td>0.076</td>
| + | |
| − | <td>0.083</td>
| + | |
| − | <td>0.081</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.088</td>
| + | |
| − | <td>0.081</td>
| + | |
| − | <td>0.057</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 4</th>
| + | |
| − | <td>0.086</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.083</td>
| + | |
| − | <td>0.075</td>
| + | |
| − | <td>0.072</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.072</td>
| + | |
| − | <td>0.082</td>
| + | |
| − | <td>0.056</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h5>Hour 6:</h5>
| + | |
| − | <table id="Abs600RawReadingsHour6">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | <th>LB + Chloro. (blank)</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 1</th>
| + | |
| − | <td>0.445</td>
| + | |
| − | <td>0.437</td>
| + | |
| − | <td>0.258</td>
| + | |
| − | <td>0.447</td>
| + | |
| − | <td>0.487</td>
| + | |
| − | <td>0.108</td>
| + | |
| − | <td>0.329</td>
| + | |
| − | <td>0.459</td>
| + | |
| − | <td>0.062</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 2</th>
| + | |
| − | <td>0.456</td> | + | |
| − | <td>0.475</td>
| + | |
| − | <td>0.261</td>
| + | |
| − | <td>0.444</td>
| + | |
| − | <td>0.518</td>
| + | |
| − | <td>0.140</td>
| + | |
| − | <td>0.324</td>
| + | |
| − | <td>0.477</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 3</th>
| + | |
| − | <td>0.473</td>
| + | |
| − | <td>0.433</td>
| + | |
| − | <td>0.270</td>
| + | |
| − | <td>0.450</td>
| + | |
| − | <td>0.524</td>
| + | |
| − | <td>0.109</td>
| + | |
| − | <td>0.353</td>
| + | |
| − | <td>0.450</td>
| + | |
| − | <td>0.063</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1 Replicate 4</th>
| + | |
| − | <td>0.475</td>
| + | |
| − | <td>0.431</td>
| + | |
| − | <td>0.254</td>
| + | |
| − | <td>0.444</td>
| + | |
| − | <td>0.535</td>
| + | |
| − | <td>0.109</td>
| + | |
| − | <td>0.328</td>
| + | |
| − | <td>0.438</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 1</th>
| + | |
| − | <td>0.456</td>
| + | |
| − | <td>0.414</td>
| + | |
| − | <td>0.229</td>
| + | |
| − | <td>0.459</td>
| + | |
| − | <td>0.444</td>
| + | |
| − | <td>0.115</td>
| + | |
| − | <td>0.112</td>
| + | |
| − | <td>0.463</td>
| + | |
| − | <td>0.060</td>
| + | |
| − | </tr> | + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 2</th>
| + | |
| − | <td>0.411</td>
| + | |
| − | <td>0.429</td>
| + | |
| − | <td>0.243</td>
| + | |
| − | <td>0.452</td>
| + | |
| − | <td>0.460</td>
| + | |
| − | <td>0.119</td>
| + | |
| − | <td>0.106</td>
| + | |
| − | <td>0.453</td>
| + | |
| − | <td>0.058</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 3</th>
| + | |
| − | <td>0.435</td>
| + | |
| − | <td>0.418</td>
| + | |
| − | <td>0.234</td>
| + | |
| − | <td>0.474</td>
| + | |
| − | <td>0.460</td>
| + | |
| − | <td>0.120</td>
| + | |
| − | <td>0.113</td>
| + | |
| − | <td>0.455</td>
| + | |
| − | <td>0.063</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2 Replicate 4</th>
| + | |
| − | <td>0.425</td>
| + | |
| − | <td>0.424</td>
| + | |
| − | <td>0.248</td>
| + | |
| − | <td>0.470</td>
| + | |
| − | <td>0.450</td>
| + | |
| − | <td>0.121</td>
| + | |
| − | <td>0.115</td>
| + | |
| − | <td>0.466</td>
| + | |
| − | <td>0.061</td>
| + | |
| − | </tr>
| + | |
| − | </table> | + | |
| − | <h3>Fluoresence per OD</h3>
| + | |
| − | <h4>µM Flourescin/OD</h4>
| + | |
| − | <h5>Hour 0:</h5>
| + | |
| − | <table id="uMFlourescinODHour0">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>0.000</td>
| + | |
| − | <td>0.078</td>
| + | |
| − | <td>0.145</td>
| + | |
| − | <td>0.059</td>
| + | |
| − | <td>0.003</td>
| + | |
| − | <td>0.386</td>
| + | |
| − | <td>0.253</td>
| + | |
| − | <td>0.203</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>0.010</td>
| + | |
| − | <td>0.039</td>
| + | |
| − | <td>0.105</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.124</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.061</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.039</td>
| + | |
| − | <td>0.105</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.124</td>
| + | |
| − | <td>0.074</td>
| + | |
| − | <td>0.061</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.051</td>
| + | |
| − | <td>0.121</td>
| + | |
| − | <td>0.048</td>
| + | |
| − | <td>0.009</td>
| + | |
| − | <td>0.141</td>
| + | |
| − | <td>0.090</td>
| + | |
| − | <td>0.068</td>
| + | |
| − | </tr> | + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>-0.009</td>
| + | |
| − | <td>0.043</td>
| + | |
| − | <td>0.088</td>
| + | |
| − | <td>0.031</td>
| + | |
| − | <td>0.006</td>
| + | |
| − | <td>0.177</td>
| + | |
| − | <td>0.101</td>
| + | |
| − | <td>0.068</td>
| + | |
| − | </tr> | + | |
| − | <tr class="yellowCells"> | + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th> | + | |
| − | <td>0.010</td>
| + | |
| − | <td>0.042</td>
| + | |
| − | <td>0.125</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | <td>0.012</td>
| + | |
| − | <td>0.179</td>
| + | |
| − | <td>0.140</td>
| + | |
| − | <td>0.037</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.037</td>
| + | |
| − | <td>0.121</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | <td>0.012</td>
| + | |
| − | <td>0.161</td>
| + | |
| − | <td>0.077</td>
| + | |
| − | <td>0.040</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>0.007</td>
| + | |
| − | <td>0.051</td>
| + | |
| − | <td>0.146</td>
| + | |
| − | <td>0.035</td>
| + | |
| − | <td>0.022</td>
| + | |
| − | <td>0.208</td>
| + | |
| − | <td>0.199</td>
| + | |
| − | <td>0.049</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h5>Hour 6:</h5>
| + | |
| − | <table id="uMFlourescinODHour6">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>0.001</td>
| + | |
| − | <td>0.036</td>
| + | |
| − | <td>0.109</td>
| + | |
| − | <td>0.030</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.119</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.030</td>
| + | |
| − | <td>0.091</td>
| + | |
| − | <td>0.025</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.058</td>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.020</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.032</td>
| + | |
| − | <td>0.089</td>
| + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.092</td>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.021</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.032</td>
| + | |
| − | <td>0.089</td>
| + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.092</td>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.021</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>0.002</td> | + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.091</td>
| + | |
| − | <td>0.015</td>
| + | |
| − | <td>0.011</td>
| + | |
| − | <td>0.085</td>
| + | |
| − | <td>0.113</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.087</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.011</td>
| + | |
| − | <td>0.081</td>
| + | |
| − | <td>0.119</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>0.002</td>
| + | |
| − | <td>0.027</td>
| + | |
| − | <td>0.096</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.011</td>
| + | |
| − | <td>0.084</td>
| + | |
| − | <td>0.118</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>0.003</td> | + | |
| − | <td>0.033</td>
| + | |
| − | <td>0.106</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.107</td>
| + | |
| − | <td>0.150</td>
| + | |
| − | <td>0.022</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h4>Net Flourescin a.u.</h4>
| + | |
| − | <h5>Hour 0:</h5>
| + | |
| − | <table id="netFluoresceinauHour0">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>0</td>
| + | |
| − | <td>20</td>
| + | |
| − | <td>40</td>
| + | |
| − | <td>21</td>
| + | |
| − | <td>1</td>
| + | |
| − | <td>61</td>
| + | |
| − | <td>40</td>
| + | |
| − | <td>32</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>5</td>
| + | |
| − | <td>16</td>
| + | |
| − | <td>31</td>
| + | |
| − | <td>16</td>
| + | |
| − | <td>1</td>
| + | |
| − | <td>49</td>
| + | |
| − | <td>32</td>
| + | |
| − | <td>24</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>5</td>
| + | |
| − | <td>15</td>
| + | |
| − | <td>31</td>
| + | |
| − | <td>18</td>
| + | |
| − | <td>3</td>
| + | |
| − | <td>50</td>
| + | |
| − | <td>32</td>
| + | |
| − | <td>24</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>-2</td>
| + | |
| − | <td>16</td>
| + | |
| − | <td>33</td>
| + | |
| − | <td>14</td>
| + | |
| − | <td>2</td>
| + | |
| − | <td>49</td>
| + | |
| − | <td>32</td>
| + | |
| − | <td>27</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>4</td>
| + | |
| − | <td>14</td>
| + | |
| − | <td>59</td>
| + | |
| − | <td>8</td>
| + | |
| − | <td>4</td>
| + | |
| − | <td>53</td>
| + | |
| − | <td>47</td>
| + | |
| − | <td>17</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| + | |
| − | <td>6</td>
| + | |
| − | <td>12</td>
| + | |
| − | <td>59</td>
| + | |
| − | <td>9</td>
| + | |
| − | <td>3</td>
| + | |
| − | <td>53</td>
| + | |
| − | <td>47</td>
| + | |
| − | <td>17</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>6</td>
| + | |
| − | <td>14</td>
| + | |
| − | <td>62</td>
| + | |
| − | <td>11</td>
| + | |
| − | <td>4</td>
| + | |
| − | <td>54</td>
| + | |
| − | <td>47</td>
| + | |
| − | <td>19</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>4</td>
| + | |
| − | <td>14</td>
| + | |
| − | <td>78</td>
| + | |
| − | <td>13</td>
| + | |
| − | <td>7</td>
| + | |
| − | <td>74</td>
| + | |
| − | <td>63</td>
| + | |
| − | <td>25</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h5>Hour 6:</h5>
| + | |
| − | <table id="netFluoresceinauHour6">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>7</td>
| + | |
| − | <td>264</td>
| + | |
| − | <td>423</td>
| + | |
| − | <td>227</td>
| + | |
| − | <td>13</td>
| + | |
| − | <td>108</td>
| + | |
| − | <td>87</td>
| + | |
| − | <td>181</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>16</td>
| + | |
| − | <td>261</td>
| + | |
| − | <td>263</td>
| + | |
| − | <td>193</td>
| + | |
| − | <td>16</td>
| + | |
| − | <td>92</td>
| + | |
| − | <td>73</td>
| + | |
| − | <td>166</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>14</td>
| + | |
| − | <td>231</td>
| + | |
| − | <td>362</td>
| + | |
| − | <td>195</td>
| + | |
| − | <td>16</td>
| + | |
| − | <td>87</td>
| + | |
| − | <td>81</td>
| + | |
| − | <td>157</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>14</td>
| + | |
| − | <td>238</td>
| + | |
| − | <td>359</td>
| + | |
| − | <td>194</td>
| + | |
| − | <td>17</td>
| + | |
| − | <td>91</td>
| + | |
| − | <td>71</td>
| + | |
| − | <td>157</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>12</td>
| + | |
| − | <td>184</td>
| + | |
| − | <td>305</td>
| + | |
| − | <td>121</td>
| + | |
| − | <td>81</td>
| + | |
| − | <td>92</td>
| + | |
| − | <td>116</td>
| + | |
| − | <td>137</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| + | |
| − | <td>15</td>
| + | |
| − | <td>191</td>
| + | |
| − | <td>316</td>
| + | |
| − | <td>128</td>
| + | |
| − | <td>86</td>
| + | |
| − | <td>97</td>
| + | |
| − | <td>113</td>
| + | |
| − | <td>139</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>14</td>
| + | |
| − | <td>187</td>
| + | |
| − | <td>325</td>
| + | |
| − | <td>127</td>
| + | |
| − | <td>85</td>
| + | |
| − | <td>94</td>
| + | |
| − | <td>116</td>
| + | |
| − | <td>138</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>18</td>
| + | |
| − | <td>235</td>
| + | |
| − | <td>392</td>
| + | |
| − | <td>152</td>
| + | |
| − | <td>107</td>
| + | |
| − | <td>127</td>
| + | |
| − | <td>160</td>
| + | |
| − | <td>175</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <div id="Net Abs 600">
| + | |
| − | <h5>Hour 0:</h5>
| + | |
| − | <table id="netAbs600Hour0">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | <td>0.015</td>
| + | |
| − | <td>0.008</td>
| + | |
| − | <td>0.008</td>
| + | |
| − | <td>0.008</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>0.025</td>
| + | |
| − | <td>0.021</td>
| + | |
| − | <td>0.015</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | <td>0.021</td>
| + | |
| − | <td>0.020</td>
| + | |
| − | <td>0.022</td>
| + | |
| − | <td>0.020</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>0.018</td>
| + | |
| − | <td>0.015</td>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>0.011</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.020</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>0.021</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.024</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.012</td>
| + | |
| − | <td>0.014</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.024</td>
| + | |
| − | <td>0.020</td>
| + | |
| − | <td>0.013</td>
| + | |
| − | <td>0.015</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.023</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>0.024</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.026</td>
| + | |
| − | <td>0.024</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.017</td>
| + | |
| − | <td>0.031</td>
| + | |
| − | <td>0.024</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>0.030</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.027</td>
| + | |
| − | <td>0.019</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.018</td>
| + | |
| − | <td>0.016</td>
| + | |
| − | <td>0.026</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <h5>Hour 6:</h5>
| + | |
| − | <table id="netAbs600Hour6">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Negative Control</th>
| + | |
| − | <th>Positive Control</th>
| + | |
| − | <th>Device 1</th>
| + | |
| − | <th>Device 2</th>
| + | |
| − | <th>Device 3</th>
| + | |
| − | <th>Device 4</th>
| + | |
| − | <th>Device 5</th>
| + | |
| − | <th>Device 6</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| + | |
| − | <td>0.383</td>
| + | |
| − | <td>0.375</td>
| + | |
| − | <td>0.196</td>
| + | |
| − | <td>0.385</td>
| + | |
| − | <td>0.425</td>
| + | |
| − | <td>0.046</td>
| + | |
| − | <td>0.267</td>
| + | |
| − | <td>0.397</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| + | |
| − | <td>0.397</td>
| + | |
| − | <td>0.416</td>
| + | |
| − | <td>0.202</td>
| + | |
| − | <td>0.385</td>
| + | |
| − | <td>0.459</td>
| + | |
| − | <td>0.081</td>
| + | |
| − | <td>0.265</td>
| + | |
| − | <td>0.418</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| + | |
| − | <td>0.410</td>
| + | |
| − | <td>0.370</td>
| + | |
| − | <td>0.207</td>
| + | |
| − | <td>0.387</td>
| + | |
| − | <td>0.461</td>
| + | |
| − | <td>0.046</td>
| + | |
| − | <td>0.290</td>
| + | |
| − | <td>0.387</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| + | |
| − | <td>0.416</td>
| + | |
| − | <td>0.372</td>
| + | |
| − | <td>0.195</td>
| + | |
| − | <td>0.385</td>
| + | |
| − | <td>0.476</td>
| + | |
| − | <td>0.050</td>
| + | |
| − | <td>0.269</td>
| + | |
| − | <td>0.379</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| + | |
| − | <td>0.396</td>
| + | |
| − | <td>0.354</td>
| + | |
| − | <td>0.169</td>
| + | |
| − | <td>0.399</td>
| + | |
| − | <td>0.384</td>
| + | |
| − | <td>0.055</td>
| + | |
| − | <td>0.052</td>
| + | |
| − | <td>0.403</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| + | |
| − | <td>0.353</td>
| + | |
| − | <td>0.371</td>
| + | |
| − | <td>0.185</td>
| + | |
| − | <td>0.394</td>
| + | |
| − | <td>0.402</td>
| + | |
| − | <td>0.061</td>
| + | |
| − | <td>0.048</td>
| + | |
| − | <td>0.395</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| + | |
| − | <td>0.372</td>
| + | |
| − | <td>0.355</td>
| + | |
| − | <td>0.171</td>
| + | |
| − | <td>0.411</td>
| + | |
| − | <td>0.397</td>
| + | |
| − | <td>0.057</td>
| + | |
| − | <td>0.050</td>
| + | |
| − | <td>0.392</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="yellowCells">
| + | |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| + | |
| − | <td>0.364</td>
| + | |
| − | <td>0.363</td>
| + | |
| − | <td>0.187</td>
| + | |
| − | <td>0.409</td>
| + | |
| − | <td>0.389</td>
| + | |
| − | <td>0.060</td>
| + | |
| − | <td>0.054</td>
| + | |
| − | <td>0.405</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| | </div> | | </div> |
| | </div> | | </div> |
| − | <h3>Fluorescence per Particle</h3>
| |
| − | <h4>MEFL/particle</h4>
| |
| − | <h5>Hour 0:</h5>
| |
| − | <table id="MEFLParticleHour0">
| |
| − | <tr>
| |
| − | <th></th>
| |
| − | <th>Negative Control</th>
| |
| − | <th>Positive Control</th>
| |
| − | <th>Device 1</th>
| |
| − | <th>Device 2</th>
| |
| − | <th>Device 3</th>
| |
| − | <th>Device 4</th>
| |
| − | <th>Device 5</th>
| |
| − | <th>Device 6</th>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| |
| − | <td>0</td>
| |
| − | <td>3.67×10<sup>3</sup></td>
| |
| − | <td>6.82×10<sup>3</sup></td>
| |
| − | <td>2.79×10<sup>3</sup></td>
| |
| − | <td>1.59×10<sup>2</sup></td>
| |
| − | <td>1.82×10<sup>4</sup></td>
| |
| − | <td>1.19×10<sup>4</sup></td>
| |
| − | <td>9.55×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| |
| − | <td>4.78×10<sup>2</sup></td>
| |
| − | <td>1.82×10<sup>3</sup></td>
| |
| − | <td>4.93×10<sup>3</sup></td>
| |
| − | <td>1.66×10<sup>3</sup></td>
| |
| − | <td>1.14×10<sup>2</sup></td>
| |
| − | <td>5.85×10<sup>3</sup></td>
| |
| − | <td>3.47×10<sup>3</sup></td>
| |
| − | <td>2.87×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| |
| − | <td>6.63×10<sup>2</sup></td>
| |
| − | <td>2.39×10<sup>3</sup></td>
| |
| − | <td>5.69×10<sup>3</sup></td>
| |
| − | <td>2.26×10<sup>3</sup></td>
| |
| − | <td>4.21×10<sup>2</sup></td>
| |
| − | <td>6.63×10<sup>3</sup></td>
| |
| − | <td>4.24×10<sup>3</sup></td>
| |
| − | <td>3.18×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| |
| − | <td>-4.34×10<sup>2</sup></td>
| |
| − | <td>2.01×10<sup>3</sup></td>
| |
| − | <td>4.15×10<sup>3</sup></td>
| |
| − | <td>1.45×10<sup>3</sup></td>
| |
| − | <td>2.81×10<sup>2</sup></td>
| |
| − | <td>8.36×10<sup>3</sup></td>
| |
| − | <td>4.78×10<sup>3</sup></td>
| |
| − | <td>3.22×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| |
| − | <td>4.55×10<sup>2</sup></td>
| |
| − | <td>1.97×10<sup>3</sup></td>
| |
| − | <td>5.87×10<sup>3</sup></td>
| |
| − | <td>1.12×10<sup>3</sup></td>
| |
| − | <td>7.96×10<sup>2</sup></td>
| |
| − | <td>9.04×10<sup>3</sup></td>
| |
| − | <td>5.91×10<sup>3</sup></td>
| |
| − | <td>2.26×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| |
| − | <td>5.51×10<sup>2</sup></td>
| |
| − | <td>1.79×10<sup>3</sup></td>
| |
| − | <td>5.87×10<sup>3</sup></td>
| |
| − | <td>1.07×10<sup>3</sup></td>
| |
| − | <td>5.51×10<sup>2</sup></td>
| |
| − | <td>8.44×10<sup>3</sup></td>
| |
| − | <td>6.60×10<sup>3</sup></td>
| |
| − | <td>1.76×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| |
| − | <td>5.97×10<sup>2</sup></td>
| |
| − | <td>1.76×10<sup>3</sup></td>
| |
| − | <td>5.69×10<sup>3</sup></td>
| |
| − | <td>1.09×10<sup>3</sup></td>
| |
| − | <td>5.62×10<sup>2</sup></td>
| |
| − | <td>7.58×10<sup>3</sup></td>
| |
| − | <td>3.62×10<sup>3</sup></td>
| |
| − | <td>1.89×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| |
| − | <td>3.18×10<sup>2</sup></td>
| |
| − | <td>2.39×10<sup>3</sup></td>
| |
| − | <td>6.90×10<sup>3</sup></td>
| |
| − | <td>1.63×10<sup>3</sup></td>
| |
| − | <td>1.04×10<sup>3</sup></td>
| |
| − | <td>9.82×10<sup>3</sup></td>
| |
| − | <td>9.40×10<sup>3</sup></td>
| |
| − | <td>2.03×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | </table>
| |
| − | <h5>Hour 6:</h5>
| |
| − | <table id="MEFLParticleHour6">
| |
| − | <tr>
| |
| − | <th></th>
| |
| − | <th>Negative Control</th>
| |
| − | <th>Positive Control</th>
| |
| − | <th>Device 1</th>
| |
| − | <th>Device 2</th>
| |
| − | <th>Device 3</th>
| |
| − | <th>Device 4</th>
| |
| − | <th>Device 5</th>
| |
| − | <th>Device 6</th>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 1</th>
| |
| − | <td>4.36×10<sup>1</sup></td>
| |
| − | <td>1.68×10<sup>3</sup></td>
| |
| − | <td>5.15×10<sup>3</sup></td>
| |
| − | <td>1.41×10<sup>3</sup></td>
| |
| − | <td>7.30×10<sup>1</sup></td>
| |
| − | <td>5.61×10<sup>3</sup></td>
| |
| − | <td>7.78×10<sup>2</sup></td>
| |
| − | <td>1.09×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 2</th>
| |
| − | <td>9.62×10<sup>1</sup></td>
| |
| − | <td>1.41×10<sup>3</sup></td>
| |
| − | <td>2.28×10<sup>3</sup></td>
| |
| − | <td>1.20×10<sup>3</sup></td>
| |
| − | <td>8.32×10<sup>1</sup></td>
| |
| − | <td>2.71×10<sup>3</sup></td>
| |
| − | <td>6.58×10<sup>2</sup></td>
| |
| − | <td>9.48×10<sup>2</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 3</th>
| |
| − | <td>8.15×10<sup>1</sup></td>
| |
| − | <td>1.49×10<sup>3</sup></td>
| |
| − | <td>4.18×10<sup>3</sup></td>
| |
| − | <td>1.20×10<sup>3</sup></td>
| |
| − | <td>8.29×10<sup>1</sup></td>
| |
| − | <td>4.52×10<sup>3</sup></td>
| |
| − | <td>6.67×10<sup>2</sup></td>
| |
| − | <td>9.69×10<sup>2</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 1, Replicate 4</th>
| |
| − | <td>8.04×10<sup>1</sup></td>
| |
| − | <td>1.53×10<sup>3</sup></td>
| |
| − | <td>4.40×10<sup>3</sup></td>
| |
| − | <td>1.20×10<sup>3</sup></td>
| |
| − | <td>8.53×10<sup>2</sup></td>
| |
| − | <td>4.35×10<sup>3</sup></td>
| |
| − | <td>6.30×10<sup>2</sup></td>
| |
| − | <td>9.89×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 1</th>
| |
| − | <td>7.24×10<sup>1</sup></td>
| |
| − | <td>1.24×10<sup>3</sup></td>
| |
| − | <td>4.31×10<sup>3</sup></td>
| |
| − | <td>7.24×10<sup>2</sup></td>
| |
| − | <td>5.04×10<sup>2</sup></td>
| |
| − | <td>3.99×10<sup>3</sup></td>
| |
| − | <td>5.33×10<sup>3</sup></td>
| |
| − | <td>8.12×10<sup>2</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 2</th>
| |
| − | <td>1.01×10<sup>2</sup></td>
| |
| − | <td>1.23×10<sup>3</sup></td>
| |
| − | <td>4.08×10<sup>3</sup></td>
| |
| − | <td>7.76×10<sup>2</sup></td>
| |
| − | <td>5.11×10<sup>2</sup></td>
| |
| − | <td>3.80×10<sup>3</sup></td>
| |
| − | <td>5.62×10<sup>3</sup></td>
| |
| − | <td>8.40×10<sup>2</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 3</th>
| |
| − | <td>8.99×10<sup>1</sup></td>
| |
| − | <td>1.26×10<sup>3</sup></td>
| |
| − | <td>4.54×10<sup>3</sup></td>
| |
| − | <td>7.38×10<sup>2</sup></td>
| |
| − | <td>5.11×10<sup>2</sup></td>
| |
| − | <td>3.94×10<sup>3</sup></td>
| |
| − | <td>5.54×10<sup>3</sup></td>
| |
| − | <td>8.41×10<sup>2</sup></td>
| |
| − | </tr>
| |
| − | <tr class="yellowCells">
| |
| − | <th class="whiteCellsImportant">Colony 2, Replicate 4</th>
| |
| − | <td>1.18×10<sup>2</sup></td>
| |
| − | <td>1.55×10<sup>3</sup></td>
| |
| − | <td>5.01×10<sup>3</sup></td>
| |
| − | <td>8.87×10<sup>2</sup></td>
| |
| − | <td>6.57×10<sup>2</sup></td>
| |
| − | <td>5.05×10<sup>3</sup></td>
| |
| − | <td>7.07×10<sup>3</sup></td>
| |
| − | <td>1.03×10<sup>3</sup></td>
| |
| − | </tr>
| |
| − | </table>
| |
| | </div> | | </div> |
| − | <div id="Colony Forming Units"> | + | <div id="cellmeasurment"> |
| − | <h2>Colony Forming Units per 0.1 OD600 <i>E. coli</i> Cultures</h2>
| + | <div class="meatSubtitle"> |
| − | <div id="coloniesPerPlate"> | + | 2. Cell Measurement |
| − | <h3>Colonies per Plate</h3>
| + | |
| − | <h4>Positive Control</h4>
| + | |
| − | <table id="coloniesPerPlatePositive">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Colony 1, Replicate 1</th>
| + | |
| − | <th>Colony 1, Replicate 2</th>
| + | |
| − | <th>Colony 1, Replicate 3</th>
| + | |
| − | <th>Colony 2, Replicate 1</th>
| + | |
| − | <th>Colony 2, Replicate 2</th>
| + | |
| − | <th>Colony 2, Replicate 3</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 3</th>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 4</th>
| + | |
| − | <td>64</td>
| + | |
| − | <td>129</td>
| + | |
| − | <td>127</td>
| + | |
| − | <td>9</td>
| + | |
| − | <td>143</td>
| + | |
| − | <td>80</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 5</th>
| + | |
| − | <td>13</td>
| + | |
| − | <td>8</td>
| + | |
| − | <td>12</td>
| + | |
| − | <td>5</td>
| + | |
| − | <td>18</td>
| + | |
| − | <td>5</td>
| + | |
| − | </tr>
| + | |
| − | </table> | + | |
| − | <h4>Negative Control</h4>
| + | |
| − | <table id="coloniesPerPlateNegative">
| + | |
| − | <tr>
| + | |
| − | <th></th>
| + | |
| − | <th>Colony 1, Replicate 1</th>
| + | |
| − | <th>Colony 1, Replicate 2</th>
| + | |
| − | <th>Colony 1, Replicate 3</th>
| + | |
| − | <th>Colony 2, Replicate 1</th>
| + | |
| − | <th>Colony 2, Replicate 2</th>
| + | |
| − | <th>Colony 2, Replicate 3</th>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 3</th>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>157</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 4</th>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>>300</td>
| + | |
| − | <td>92</td>
| + | |
| − | <td>259</td>
| + | |
| − | <td>5</td>
| + | |
| − | <td>3</td>
| + | |
| − | </tr>
| + | |
| − | <tr class="blueCells">
| + | |
| − | <th class="whiteCellsImportant">Dilution 5</th>
| + | |
| − | <td>41</td>
| + | |
| − | <td>33</td>
| + | |
| − | <td>14</td>
| + | |
| − | <td>11</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>0</td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| | </div> | | </div> |
| − | <div id="colonyFormingUnitspermL"> | + | <div class="meatMeat"> |
| − | <h3>Colony Forming Units per mL</h3> | + | Plate Reader Raw Measurement |
| − | <h4>Positive Control</h4> | + | <br> |
| − | <table id="colonyFormingUnitspermlPoistive"> | + | Fluorescence Raw Readings |
| − | <tr> | + | <br> |
| − | <th></th>
| + | Hour 0: |
| − | <th>Colony 1, Replicate 1</th>
| + | <br> |
| − | <th>Colony 1, Replicate 2</th>
| + | <div class="center"> |
| − | <th>Colony 1, Replicate 3</th>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab6.png" alt="waluigi time"> |
| − | <th>Colony 2, Replicate 1</th>
| + | </div> |
| − | <th>Colony 2, Replicate 2</th>
| + | Hour 6: |
| − | <th>Colony 2, Replicate 3</th>
| + | <br> |
| − | </tr>
| + | <div class="center"> |
| − | <tr class="yellowCells">
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab7.png" alt="waluigi time"> |
| − | <th class="whiteCellsImportant">Dilution 3</th>
| + | </div> |
| − | <td>-</td>
| + | <br> |
| − | <td>-</td>
| + | Abs600 Raw Readings |
| − | <td>-</td>
| + | <br> |
| − | <td>-</td>
| + | Hour 0: |
| − | <td>-</td>
| + | <br> |
| − | <td>-</td>
| + | <div class="center"> |
| − | </tr>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab8.png" alt="waluigi time"> |
| − | <tr class="yellowCells">
| + | </div> |
| − | <th class="whiteCellsImportant">Dilution 4</th>
| + | Hour 6: |
| − | <td>5.12×10<sup>7</sup></td>
| + | <br> |
| − | <td>1.03×10<sup>8</sup></td>
| + | <div class="center"> |
| − | <td>1.02×10<sup>8</sup></td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab9.png" alt="waluigi time"> |
| − | <td>7.20×10<sup>6</sup></td>
| + | </div> |
| − | <td>1.14×10<sup>8</sup></td>
| + | <br> |
| − | <td>6.40×10<sup>7</sup></td>
| + | Fluorescence per OD |
| − | </tr>
| + | <br> |
| − | <tr class="yellowCells">
| + | µM Flourescin/OD |
| − | <th class="whiteCellsImportant">Dilution 5</th>
| + | <br> |
| − | <td>1.04×10<sup>8</sup></td>
| + | Hour 0: |
| − | <td>6.40×10<sup>7</sup></td>
| + | <br> |
| − | <td>9.60×10<sup>7</sup></td>
| + | <div class="center"> |
| − | <td>4.00×10<sup>7</sup></td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab10.png" alt="waluigi time"> |
| − | <td>1.44×10<sup>8</sup></td>
| + | </div> |
| − | <td>4.00×10<sup>7</sup></td>
| + | Hour 6: |
| − | </tr>
| + | <br> |
| − | </table> | + | <div class="center"> |
| − | <h4>Negative Control</h4> | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab11.png" alt="waluigi time"> |
| − | <table id="colonyFormingUnitspermlPositive"> | + | </div> |
| − | <tr> | + | <br> |
| − | <th></th>
| + | Net Fluorescin a.u. |
| − | <th>Colony 1, Replicate 1</th>
| + | <br> |
| − | <th>Colony 1, Replicate 2</th>
| + | Hour 0: |
| − | <th>Colony 1, Replicate 3</th>
| + | <br> |
| − | <th>Colony 2, Replicate 1</th>
| + | <div class="center"> |
| − | <th>Colony 2, Replicate 2</th>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab12.png" alt="waluigi time"> |
| − | <th>Colony 2, Replicate 3</th>
| + | </div> |
| − | </tr>
| + | Hour 6: |
| − | <tr class="yellowCells">
| + | <br> |
| − | <th class="whiteCellsImportant">Dilution 3</th>
| + | <div class="center"> |
| − | <td>-</td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab13.png" alt="waluigi time"> |
| − | <td>-</td>
| + | </div> |
| − | <td>-</td>
| + | Hour 0: |
| − | <td>1.26×10<sup>7</sup></td>
| + | <br> |
| − | <td>-</td>
| + | <div class="center"> |
| − | <td>-</td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab14.png" alt="waluigi time"> |
| − | </tr>
| + | </div> |
| − | <tr class="yellowCells">
| + | Hour 6: |
| − | <th class="whiteCellsImportant">Dilution 4</th>
| + | <br> |
| − | <td>-</td>
| + | <div class="center"> |
| − | <td>-</td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab15.png" alt="waluigi time"> |
| − | <td>7.36×10<sup>7</sup></td>
| + | </div> |
| − | <td>2.07×10<sup>8</sup></td>
| + | <br> |
| − | <td>4.00×10<sup>6</sup></td>
| + | Fluorescence per Particle |
| − | <td>2.40×10<sup>6</sup></td>
| + | <br> |
| − | </tr>
| + | MEFL/Particle |
| − | <tr class="yellowCells">
| + | <br> |
| − | <th class="whiteCellsImportant">Dilution 5</th>
| + | Hour 0: |
| − | <td>3.28×10<sup>8</sup></td>
| + | <br> |
| − | <td>2.64×10<sup>8</sup></td>
| + | <div class="center"> |
| − | <td>1.12×10<sup>8</sup></td>
| + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab16.png" alt="waluigi time"> |
| − | <td>8.80×10<sup>7</sup></td>
| + | </div> |
| − | <td>0</td>
| + | Hour 6: |
| − | <td>0</td>
| + | <br> |
| − | </tr>
| + | <div class="center"> |
| − | </table> | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab17.png" alt="waluigi time"> |
| | + | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| − | <div id="Misc. Info"> | + | <div id="cfus"> |
| − | <h2>Miscellaneous Information</h2> | + | <div class="meatSubtitle"> |
| − | <p>The 96 well plate we used was clear. Some of the flourescence data might be affected due to bleeding of flourescence from certain wells to another.</p> | + | 3. CFUs per 0.1 OD600 <i>E. coli</i> Cultures |
| | + | </div> |
| | + | <div class="meatMeat"> |
| | + | Colonies Per Plate |
| | + | <br> |
| | + | Positive Control |
| | + | <br> |
| | + | <div class="center"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab18.png" alt="waluigi time"> |
| | + | </div> |
| | + | Negative Control |
| | + | <br> |
| | + | <div class="center"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab19.png" alt="waluigi time"> |
| | + | </div> |
| | + | <br> |
| | + | Colony Forming Units per mL |
| | + | <br> |
| | + | Positive Control |
| | + | <br> |
| | + | <div class="center"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab20.png" alt="waluigi time"> |
| | + | </div> |
| | + | Negative Control |
| | + | <br> |
| | + | <div class="center"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab21.png" alt="waluigi time"> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <div id="Discussion"> |
| | + | <div class="meatSubtitle"> |
| | + | Discussion |
| | + | </div> |
| | + | <div class="meatMeat"> |
| | + | Our results indicate an increase in absorbance and fluorescence from 0 hours to 6 hours which indicates that there were more cells present after 6 hours at growth which contributed to a higher fluorescence. In particular, Test Device 1 had the highest fluorescence at both time points. Our positive and negative controls worked as intended and there was very little fluorescence detected from the negative control despite high absorbance indicating the presence of many cells. On average, there was a slight decrease in average fluorescence per molecule and average fluorescence per OD when comparing the 0 hour time point to the 6 hour time point, but it was mainly consistent. The individual cells did not become more or less fluorescent based on cellular concentration in the media. |
| | + | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| − |
| |
| − | <div class="copySTRIKE">
| |
| − | <div class="onTopOfCopystrike"></div>
| |
| − | <div class="bottomStuff">
| |
| − | <p>yeah we realize this is not so pretty. we're working on it.</p>
| |
| − | <p>contact us at <a href="mailto:umarylandigem@gmail.com">umarylandigem@gmail.com</a></p>
| |
| − | </div>
| |
| − | </div>
| |
| | </body> | | </body> |
| | | | |
Hour 6:
Hour 6:
Hour 6:
Hour 6:
Hour 0:
Hour 6:
Hour 6:
Negative Control
Negative Control
