(Prototype team page) |
YuhangYang (Talk | contribs) (final) |
||
Line 1: | Line 1: | ||
{{SSTi-SZGD}} | {{SSTi-SZGD}} | ||
− | + | {{SSTi-SZGD/Header}} | |
+ | <html lang="en"> | ||
+ | <head> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:SSTi-SZGD/css/Parts?action=raw&ctype=text/css"/> | ||
+ | <script src="https://2018.igem.org/Team:SSTi-SZGD/js/Parts?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script> | ||
+ | </head> | ||
+ | <body> | ||
+ | |||
+ | |||
+ | <!--page_content--> | ||
+ | <div class="page_content"> | ||
+ | |||
+ | <!--Banner--> | ||
+ | <div class="Banner"></div> | ||
+ | |||
+ | <!--Content--> | ||
+ | <div class="container Content"> | ||
+ | |||
+ | <!--Overview--> | ||
+ | <section class="Overview col-xs-12 col-md-10"> | ||
+ | |||
+ | <div class="title">Overview<!--{cn}概述--></div> | ||
+ | |||
+ | <div class="content abstract"> | ||
+ | This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible. | ||
+ | <!--{cn}今年,我们共提交了7个新的部分,包括4个基本部分和3个综合部分。我们利用BGI中国公司今年8月推出的特殊基因合成产品来合成我们的基础部件,分别合成了编码序列、RBS、启动子和信号肽等基本部分。采用聚合酶链反应(PCR)、限制性内切酶连接和3a装配相结合的方法制备复合构件。所有提交的部件都与RFC 10兼容。--> | ||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | <!--All parts--> | ||
+ | <section id="All_parts" class="All_parts col-xs-12 col-md-10"> | ||
+ | |||
+ | <div class="subtitle"> | ||
+ | <span>All parts<!--{cn}所有部分--></span> | ||
+ | </div> | ||
+ | |||
+ | <div class="content table-responsive"> | ||
+ | |||
+ | <table class="table table-bordered"> | ||
+ | |||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Name<!--{cn}名称--></th> | ||
+ | <th>Type<!--{cn}类型--></th> | ||
+ | <th>Description<!--{cn}描述--></th> | ||
+ | <th>Designer<!--{cn}设计者--></th> | ||
+ | <th>Length<!--{cn}长度(bp)--></th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>1254</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Signal peptide<!--{cn}信号肽--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>99</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白(58 Kda)--></td> | ||
+ | <td>Huimin Li<!--{cn}李慧敏--></td> | ||
+ | <td>1470</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td> | ||
+ | <td>Huimin Li<!--{cn}李慧敏--></td> | ||
+ | <td>1686</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593006">BBa_K2593006</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator<!--{cn}水蛭透明质酸水解酶具有诱导启动子,RBS,一个输出信号肽,6His和终止子--></td> | ||
+ | <td>Yongtao Lin<!--{cn}林永涛--></td> | ||
+ | <td>1962</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593007">BBa_K2593007</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator.<!--{cn}具有诱导启动子、RBS和终止子的透明质酸合成酶蛋白--></td> | ||
+ | <td>Yongtao Lin<!--{cn}林永涛--></td> | ||
+ | <td>2672</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593008">BBa_K2593008</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator.<!--{cn}UDP-葡萄糖脱氢酶和UDP-果糖焦磷酸化酶具有组成性启动子RBS和终止子--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>2796</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | <!--Basic part--> | ||
+ | <section id="Basic_part" class="Basic_part col-xs-12 col-md-10"> | ||
+ | |||
+ | <div class="subtitle"> | ||
+ | <span>Basic parts<!--{cn}基本配件--></span> | ||
+ | </div> | ||
+ | |||
+ | <div class="content table-responsive"> | ||
+ | |||
+ | <table class="table"> | ||
+ | |||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Name<!--{cn}名称--></th> | ||
+ | <th>Type<!--{cn}类型--></th> | ||
+ | <th>Description<!--{cn}描述--></th> | ||
+ | <th>Designer<!--{cn}设计者--></th> | ||
+ | <th>Length<!--{cn}长度(bp)--></th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>1254</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Signal peptide<!--{cn}信号肽--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>99</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白(58 Kda)--></td> | ||
+ | <td>Huimin Li<!--{cn}李慧敏--></td> | ||
+ | <td>1470</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td> | ||
+ | <td>coding<!--{cn}编码--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td> | ||
+ | <td>Huimin Li<!--{cn}李慧敏--></td> | ||
+ | <td>1686</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | <!--Composite part--> | ||
+ | <section id="Composite_part" class="Composite_part col-xs-12 col-md-10"> | ||
+ | |||
+ | <div class="subtitle"> | ||
+ | <span>Composite parts<!--{cn}复合零件--></span> | ||
+ | </div> | ||
+ | |||
+ | <div class="content table-responsive"> | ||
+ | |||
+ | <table class="table"> | ||
+ | |||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Name<!--{cn}名称--></th> | ||
+ | <th>Type<!--{cn}类型--></th> | ||
+ | <th>Description<!--{cn}描述--></th> | ||
+ | <th>Designer<!--{cn}设计者--></th> | ||
+ | <th>Length<!--{cn}长度(bp)--></th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593006">BBa_K2593006</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator.<!--{cn}水蛭透明质酸水解酶具有诱导启动子,RBS,一个输出信号肽,6His和终止子--></td> | ||
+ | <td>Yongtao Lin<!--{cn}林永涛--></td> | ||
+ | <td>1962</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593007">BBa_K2593007</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator.<!--{cn}具有诱导启动子、RBS和终止子的透明质酸合成酶蛋白--></td> | ||
+ | <td>Yongtao Lin<!--{cn}林永涛--></td> | ||
+ | <td>2672</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2593008">BBa_K2593008</a></td> | ||
+ | <td>composite<!--{cn}综合--></td> | ||
+ | <td>UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator.<!--{cn}UDP-葡萄糖脱氢酶和UDP-果糖焦磷酸化酶具有组成性启动子RBS和终止子--></td> | ||
+ | <td>Hong Chen<!--{cn}陈红--></td> | ||
+ | <td>2796</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | <!--Improved part--> | ||
+ | <section id="Improved_part" class="Improved_part col-xs-12 col-md-10"> | ||
+ | |||
+ | <div class="subtitle"> | ||
+ | <span>Improved parts<!--{cn}改进部分--></span> | ||
+ | </div> | ||
+ | |||
+ | <div class="content abstract"> | ||
+ | <p> | ||
+ | In our project, we added to the characterization of <!--{cn}在我们的项目中,我们添加到现有的--> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K1469009">an existing Biobrick<!--{cn}生物砖块--></a> | ||
+ | , design by Philip Hartz from iGEM Saarland 2014. Our P43-tuaD-gtaB-T1 composite part was designed based on two BioBrick parts <!--{cn},设计由菲利普·哈茨从萨尔iGEM大赛2014年的表征。我们的P43-tuaD-gtaB-T1复合部件是基于两个BioBrick组件设计--> | ||
+ | ( <a href="http://parts.igem.org/Part:BBa_K1469002">BBa_K1469002</a> | ||
+ | and | ||
+ | <a href="http://parts.igem.org/Part:BBa_K1469005">BBa_K1469005</a>) created by Saarland.<!--{cn}通过萨尔设计的--></a> | ||
+ | For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant <!--{cn}。例如,将tuad-gtab作为操纵子的共同表达作为控制下的一个本构启动子以至于重组--> | ||
+ | <i>B. Subtilis 168<!--{cn}枯草芽孢杆菌168--></i> | ||
+ | produces elevated level of HA.<!--{cn}产生较高水平的透明质酸。--> | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </body> | ||
+ | </html> | ||
− | + | {{SSTi-SZGD/btn}} | |
− | + | {{SSTi-SZGD/Footer}} | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + |
Latest revision as of 14:21, 6 November 2018
Overview
This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
All parts
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K2593001 | coding | Hyaluronic acid synthase protein | Hong Chen | 1254 |
BBa_K2593003 | coding | Signal peptide | Hong Chen | 99 |
BBa_K2593004 | coding | Leech Hyaluronic acid hydrolase protein (58KDa) | Huimin Li | 1470 |
BBa_K2593009 | coding | Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus | Huimin Li | 1686 |
BBa_K2593006 | composite | Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator | Yongtao Lin | 1962 |
BBa_K2593007 | composite | Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. | Yongtao Lin | 2672 |
BBa_K2593008 | composite | UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. | Hong Chen | 2796 |
Basic parts
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K2593001 | coding | Hyaluronic acid synthase protein | Hong Chen | 1254 |
BBa_K2593003 | coding | Signal peptide | Hong Chen | 99 |
BBa_K2593004 | coding | Leech Hyaluronic acid hydrolase protein (58KDa) | Huimin Li | 1470 |
BBa_K2593009 | coding | Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus | Huimin Li | 1686 |
Composite parts
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K2593006 | composite | Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator. | Yongtao Lin | 1962 |
BBa_K2593007 | composite | Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. | Yongtao Lin | 2672 |
BBa_K2593008 | composite | UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. | Hong Chen | 2796 |
Improved parts
In our project, we added to the characterization of an existing Biobrick , design by Philip Hartz from iGEM Saarland 2014. Our P43-tuaD-gtaB-T1 composite part was designed based on two BioBrick parts ( BBa_K1469002 and BBa_K1469005) created by Saarland. For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant B. Subtilis 168 produces elevated level of HA.
-
- Application
- Applied Design
- Entrepreneurship
- Demonstrate
-
- Human Practices
- Integrated Human Practices
- Public Engagement & Out Reach
- Awards
-
- Acknowledgements
- Team
- Collaborations
- Attributions
Copyright © 2018 SSTi-SZGD All Rights Reserved.