Difference between revisions of "Team:JMU Wuerzburg/Description"
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| − | </head> | + | <title>iGEM Würzburg, Project Description</title> |
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| − | <h2>Development of a qPCR-based rapid testen system</h2> | + | <body> |
| − | <p>Recently, fast qPCR and RTqPCR diagnostical tests detecting pathogens in patient-derived samples have been developed. These tests comprise all required steps from genome purification to the result analyses in a "single tube" reaction. Our aim is to construct a test system that is adaptable on various pathogens. At first we design primers and probes by analysing all published sequences of the respective pathogens with bioinformatic tools. After that we develop our rapid tests detecting Noroviruses and the malaria-causing pathogens Plasmodium. Our test system is evaluated and optimised using synthesised templates. In a second step, we use inactivated patient-derived samples to confirm selectivity and specificity of our tests. | + | <h2>Development of a qPCR-based rapid testen system</h2> |
| − | </p> | + | <p>Recently, fast qPCR and RTqPCR diagnostical tests detecting pathogens in patient-derived samples have been developed. These tests comprise all required steps from genome purification to the result analyses in a "single tube" reaction. Our aim is to construct a test system that is adaptable on various pathogens. At first we design primers and probes by analysing all published sequences of the respective pathogens with bioinformatic tools. After that we develop our rapid tests detecting Noroviruses and the malaria-causing pathogens Plasmodium. Our test system is evaluated and optimised using synthesised templates. In a second step, we use inactivated patient-derived samples to confirm selectivity and specificity of our tests. |
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Revision as of 21:55, 15 July 2018
Development of a qPCR-based rapid testen system
Recently, fast qPCR and RTqPCR diagnostical tests detecting pathogens in patient-derived samples have been developed. These tests comprise all required steps from genome purification to the result analyses in a "single tube" reaction. Our aim is to construct a test system that is adaptable on various pathogens. At first we design primers and probes by analysing all published sequences of the respective pathogens with bioinformatic tools. After that we develop our rapid tests detecting Noroviruses and the malaria-causing pathogens Plasmodium. Our test system is evaluated and optimised using synthesised templates. In a second step, we use inactivated patient-derived samples to confirm selectivity and specificity of our tests.