Difference between revisions of "Team:JNFLS"
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<h4> Abstract </h4> | <h4> Abstract </h4> | ||
| − | <p>Development of a biosensor for detecting HCV antigen (C+E1+E2) by the nucleic acid aptamer. In the blood test of blood donation without compensation, the antibodies and RNA detection are usually used for HCV, but these detections are insufficient. Nucleic acid aptamer has been widely used for its specificity. In this project, the nucleic acid aptamer is used to detect HCV antigen, and the specific detection of trace HCV antigen could be realized by the signal amplification, which has great significance to shorten the window period in clinic transfusion. HCV C and HCV C + E1 + E2 genes are cloned respectively into secretory eukaryotic expression vectors, transfected into eukaryotic cells, and collected secreted HCV virus-like particles (VLP). At the same time, the ssDNA aptamer library is constructed. The length of nucleotide insertion is 40nt, and the storage capacity is about 106. HCV-VLP is used to screen the nucleic acid aptamer specifically bound to HCV-VLP by SELEX technology. Using the competing reaction of the target antigen, the adapter sequence, padlock probe and complementary sequence of the adapter, a highly sensitive fluorescent adapter sensor is developed based on the rolling circle replication. When there is no target antigen, the complementary sequence binders with aptamer probe instead of the padlock probe, which triggers rolling circle amplification reaction. Whereas when the aptamer-probe binds with the target antigen, the complementary sequence hybridizes with the padlock probe. Under the action of DNA ligase, the padlock probe is further cyclized and a rolling circle amplification occurs under the action of | + | <p>Development of a biosensor for detecting HCV antigen (C+E1+E2) by the nucleic acid aptamer. In the blood test of blood donation without compensation, the antibodies and RNA detection are usually used for HCV, but these detections are insufficient. Nucleic acid aptamer has been widely used for its specificity. In this project, the nucleic acid aptamer is used to detect HCV antigen, and the specific detection of trace HCV antigen could be realized by the signal amplification, which has great significance to shorten the window period in clinic transfusion. HCV C and HCV C + E1 + E2 genes are cloned respectively into secretory eukaryotic expression vectors, transfected into eukaryotic cells, and collected secreted HCV virus-like particles (VLP). At the same time, the ssDNA aptamer library is constructed. The length of nucleotide insertion is 40nt, and the storage capacity is about 106. HCV-VLP is used to screen the nucleic acid aptamer specifically bound to HCV-VLP by SELEX technology. Using the competing reaction of the target antigen, the adapter sequence, padlock probe and complementary sequence of the adapter, a highly sensitive fluorescent adapter sensor is developed based on the rolling circle replication. When there is no target antigen, the complementary sequence binders with aptamer probe instead of the padlock probe, which triggers rolling circle amplification reaction. Whereas when the aptamer-probe binds with the target antigen, the complementary sequence hybridizes with the padlock probe. Under the action of DNA ligase, the padlock probe is further cyclized and a rolling circle amplification occurs under the action of DNA polymerase. By designing different aptamer sequences and related nucleic acid sequences, the sensing system can be used as a general method to detect other targets antigen. </p> |
</div> | </div> | ||
Revision as of 14:25, 25 July 2018
Welcome to iGEM 2018!
Your team has been approved and you are ready to start the iGEM season!
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Abstract
Development of a biosensor for detecting HCV antigen (C+E1+E2) by the nucleic acid aptamer. In the blood test of blood donation without compensation, the antibodies and RNA detection are usually used for HCV, but these detections are insufficient. Nucleic acid aptamer has been widely used for its specificity. In this project, the nucleic acid aptamer is used to detect HCV antigen, and the specific detection of trace HCV antigen could be realized by the signal amplification, which has great significance to shorten the window period in clinic transfusion. HCV C and HCV C + E1 + E2 genes are cloned respectively into secretory eukaryotic expression vectors, transfected into eukaryotic cells, and collected secreted HCV virus-like particles (VLP). At the same time, the ssDNA aptamer library is constructed. The length of nucleotide insertion is 40nt, and the storage capacity is about 106. HCV-VLP is used to screen the nucleic acid aptamer specifically bound to HCV-VLP by SELEX technology. Using the competing reaction of the target antigen, the adapter sequence, padlock probe and complementary sequence of the adapter, a highly sensitive fluorescent adapter sensor is developed based on the rolling circle replication. When there is no target antigen, the complementary sequence binders with aptamer probe instead of the padlock probe, which triggers rolling circle amplification reaction. Whereas when the aptamer-probe binds with the target antigen, the complementary sequence hybridizes with the padlock probe. Under the action of DNA ligase, the padlock probe is further cyclized and a rolling circle amplification occurs under the action of DNA polymerase. By designing different aptamer sequences and related nucleic acid sequences, the sensing system can be used as a general method to detect other targets antigen.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples: