• Week 24
• Week 25
• Week 26

Week 24 (June 10 - June 16)

• 

  Wet Lab Overview

  First day in lab was used for creating media of different types and concentrations for the species to be grown on for two reasons:

  1. To illustrate an examine the growthrate (for modelling)

  2. To multiply our pure species for use in liquid media.

  We used the media created in the "PDB (or PDA) and MEA media for species" protocol. It was also the plan to create Vogel's medium as well as a mineral salt medium as certain species had shown in previous study to grow well. However, we did not have the proper materials to create those two and discarded the idea. We also came to this conclusion as we were recommended not to spend time on specific media just yet.
  All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous. The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fumehood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.

Week 25 (June 17 - June 23)

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  Wet Lab Overview

  20/6/2018
  Pleuratus ostreatus was re-inoculated on the solid agar plates by placement of around 8 mycelia "balls" under a fumehood and left at 30 degrees Celsius. As to save time, fewer plates were inoculated and a YBD plate was included.
  
  Today, we had gotten another species, Schizophyllum commune, in two forms: a wildtype as well as a mutant (Δsc3) which were also inoculated on PDB, MEA and YBD plates.
  
  22/6/2018
  First sign of growth in our species were showing and pictures were taken of every plate as to count pixels and indicate the growth rate. Pictures were taken every morning for 5 days.

• 

  Dry Lab Overview

  All members are now full time on this iGEM project!
  This week we're working on completing the Poisson Hidden Markov model (HMM).
  The forms for the wet lab team's experiments is almost done and we hope to finish the welding so we can move on to test some of the physical properties.
  
  The last goal of the week is to find a fitting model for the mycelia growth and interactions and discuss the link to the wet lab experiments.

Week 26 (June 24 - July 1)

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  Wet Lab Overview

  26/6/2018
  Aspergillus oryzae was received and inoculated on MEA, PDB and YBD plates (5 days of photographs will follow this species growth in the same manner as the other species).
  Today we decided that our species had grown enough and the samples were then removed and stored at a cooler temperature excluding those used for later liquid media:

• 

  Dry Lab Overview

  All members is now full time on this iGEM project!