Team:DTU-Denmark/Experiments

• Project
  • Description
  • Design
  • InterLab
  • Results
  • Choice of Organism
  • amilCP Expression
  • melA Expression
  • nat1 Expression
  • Ganoderma Protoplastation
  • Demonstrate
• Modeling
  • Model
  • Growth Modeling
  • Design of Experiments
  • Modeling the Design
  • Structural Integrity
  • Hardware
• Parts
• Journal
  • Notebook
  • Experiments
• 
• People
  • Team Members
  • Attributions
• Human Practices
  • Human Practices
  • Education and Engagement
• Collaboration
  • Collaborations
  • BioBrick Tutorial
• Safety

Hyphae Hackers

Project

Description Design InterLab Results Choice of Organism amilCP Expression melA Expression nat1 Expression Ganoderma Protoplastation Demonstrate

Modeling

Model Growth Modeling Design of Experiments Modeling the Design Structural Integrity Hardware

Parts Overview Journal

Notebook Experiments

People

Team Members Attributions

Safety Human Practices

Human Practices Education & Engagement

Collaborations

Collaborations BioBrick Tutorial

Experiments

More details here

All media was prepared before 11 am to be autoclaved. The actual weights of the different media was:

MEA Media
Materials	Weight (g)	Weight (g)	Weight (g)
Malt extract	47.6	49.9	52.5
ZnSO4·7H2O	0.01	0.012	0.01
CuSO4·5H2O	0.05	0.049	0.051
Agar	14.99	14.99	15.04

PDB Media
Potato dextrose extract	Weight (g)	Weight (g)	Weight (g)
Malt extract	19.54	39.12	58.56
ZnSO4·7H2O	0.012	0.012	0.0107
CuSO4·5H2O	0.0056	0.0053	0.0049
Agar	15.02	15.04	15.04

All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fume hood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.