Team:Kyoto/Safety

Safety






1) Safe project design
Basic Parts
Composite Parts
2) Safe lab work
primer namesequenceLengthTmGC%DesignerManufacturer
tropomyosinFGATCGAGAAGGACAACGCCC206560FukudaMacrogen Japan Corp
IK107cccgccgccaccatggaggcggccgcaaaatcagg359471YoshimotoMacrogen Japan Corp
3) Safe shipment
3-1 Kit
NameSupplier
Wizard® SV Gel and PCRPromega
FastGene™Plasmid Mini KitNIPPON Genetics Co.,Ltd
4-1 Miniprep

Minipreps were performed using FastGene™Plasmid Mini Kit and and Wizard® Plus SV Minipreps DNA Purification System according to the manufacturer's protocols.

4-17 Soaking

We followed this paper as for soaking.
https://link.springer.com/content/pdf/10.1007%2Fs10658-012-0035-0.pdf





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