Part Improvement
We improved the part BBa_0430, an EYFP output device, by joining it with a constitutively expressed (by ptet, BBa_R0040) ECFP gene, to be used as a control for normalization of the output measurement, as this control gene would be subject to the same influences as the reporter gene (eg. cell density, plasmid copy number, metabolic load, environment properties). This method was used following the efforts of Rudge et al. on characterizing intrinsic promoter properties.
This fluorescent protein pair is good for this type of measurement due to their spectral separation. In our experiments, we used excitation/emission values of 430/480 for CFP and 500/530 for YFP.
