Team:Uppsala/Transcriptomics/cDNA Conversion
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cDNA Conversion
What is cDNA synthesis and why is it needed?
cDNA stands for complementary DNA, and can be described as a double stranded DNA that is made with RNA as template. Our ultimate goal is the sequence the RNA that we have extracted - and with our sequencing method of choice, we need to turn the RNA back into DNA for it to be read properly. To do this, we use a technique known as reverse transcription to read and copy the RNA contents onto a newly made DNA strand, with no genetic information lost!