Team:TUDelft/Parts/CompositeParts

Composite Parts

When constructing our composite BioBrick, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. Since our fusion protein BBa_K2643000 consists of the dxCas9, linker and Tn5 coding sequences this part is registered as composite part. The basic parts comprising the our fusion protein composite part are documented separately and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. We created a part collection comprised of 6 basic parts encoding for different EPO coding sequences, 2 basic parts encoding for the required components for targeted sequencing and 1 composite part: our fusion protein. All BioBricks are submitted with elaborate characterisation so the user may choose the best protein for their project accordingly. Our favourite basic part is BBa_K2643001 and our favourite composite part is BBa_K2643000.

Table 1. BioBrick Parts submitted by TU Delft iGEM 2018 team. All BioBricks were constructed by the team members involved in wetlab: Susan Bouwmeester, Kavish Kohabir, Venda Mangkusaputra, Timmy Paez, Lisbeth Schmidtchen and Nicole Bennis

Fave Part	Name	Type	Description	Designer
♥	BBa_K2643000	Composite	dxCas9-linker-Tn5 fusion protein (HIS Tag)	TU Delft 2018