Team:Aix-Marseille/Experiments
Experiments
Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.
Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration
Beauveria Bassiana :
We did some research on Beavearia bassiana fungus because it's an entomopathogenic fungus used during years in agriculture, especially in Canada. [1]
So, we wanted to use this fungus to fight bed bugs which invade our homes. We selected and bought a Beauveria sample.
First of all, we grec Beauveria bassiana fungus on three different kind of culture medium : 1. LB with an antibiotic Cm50 2. Sabouraud with Cm 50 3. Sabouraud with collagen and Cm 50
We made a cascade dilution and let it grow at three températures : 25°C, 30°C and 37°C.
Secondly, we have tested Beauveria Sample on garden bugs with 4g of fungus sample diluted in 40ml of Tween 0,05% to confirmed that it was indeed Beauveria bassiana. We infected them by soaking and observed them during 2 weeks. Unfortunatly, results obtained were inconclusive because we have problems to keep garden bugs alive.
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So, we decided to test our Beauveria Bassiana’s sample on bed bugs in real conditions.