Team:Oxford/protocols

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Protocols

We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.

Competent cells

Culturing cells

Colony PCR

Dilutions- gBlocks/ primers

Digests (DNA)

Gel preparation

Gel loading

Gel analysis and extraction

Ligation (DNA)

oePCR

PCR

Transformation