Protocols
We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.
Competent cells
Culturing cells
Colony PCR
Dilutions- gBlocks/ primers
![Forest](https://static.igem.org/mediawiki/2018/1/10/T--Oxford--Digestionpng.png)
Digests (DNA)
Gel preparation
![Forest](https://static.igem.org/mediawiki/2018/2/2d/T--Oxford--Gelelectrophoresis.png)
Gel loading
Gel analysis and extraction
Ligation (DNA)
![Forest](https://static.igem.org/mediawiki/2018/8/85/T--Oxford--oePCR.png)
oePCR
![Forest](https://static.igem.org/mediawiki/2018/e/ed/T--Oxford--PCR.png)
PCR
![Forest](https://static.igem.org/mediawiki/2018/4/45/T--Oxford--Transformation.png)
Transformation