Team:UMaryland/BCmodel
Bacterial Cellulose Modeling
How fast can PETase CBD degrade a bottle with cellulose binding?
A 500mL PET water bottle from a common brand weighs 4 grams, is 23 centimeters tall, and has a diameter of 7 cm. If we flatten this bottle out into a sheet, its surface area will be roughly 1000 square centimeters. This sheet will be covered by sheets of bacterial cellulose on either side.
Bacterial cellulose creates a "sandwich" to maximize PETase contact with the surface
CBD has a dissociation constant of Kd= 0.038uM. Thus, its maximum binding capacity, Cmax, is 0.43uMol per gram cellulose.
© University of Maryland 2018
If cellulose has a surface area of 1000 square centimeters, working thickness of 0.1mm, and a density of 1.5 grams per cubic centimeter, the total mass of cellulose is about 15g. Thus, in a 1 L container, the maximum CBD protein binding concentration is 0.43uMol/g*15g/L = about 6.5uM protein
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umarylandigem@gmail.com
Biology - Psychology Building
4094 Campus Dr, College Park, MD 20742
© University of Maryland 2018
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