Team:Uppsala/Protocol

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Protocols

Below you can find the protocols we have been using when conducting our experiments in the lab

Worm Culturing

• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG

Transcriptomics

• TotalRNA purification protocol
• rRNA depletion protocol
• Poly(A)-tailing protocol
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG

Reporter System

• UnaG Transformation and Extraction
• Simple Chromoprotein Visibility Analysis Protocol
• Horse Feces Analysis Using a Plate Reader

Phage Display

• Panning/titering/amplification
• Phage dna for sequencing
• Original protocol supplied by New England Biolabs https://static.igem.org/mediawiki/2018/0/0c/T--Uppsala--manualE8100.pdf
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG

Improve AmilCP

• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG
• Link for protocol to UnaG

Notebook