Team:FJNU-China/Composite Part
Parts
| Part Number | Type | Part | Description | Length(bp) |
|---|---|---|---|---|
| BBa_K2570011 | Composite | dsrA+lacI+PlacI+hns+ProU+GFP | In our project, we combined temperature promoter dsrA and salt-concentration promoter ProU as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA. | 3400 |
| BBa_K2570009 | Composite | araC＋atoD＋atoA＋adhE2 | This is a composite part of degrading main unpleasant axilla odors E-3M2H(E-3-methyl-3-hexenoic acid). | 5139 |
| BBa_K2570010 | Composite | Aro10+PAR+TyrB | Production of 2-PE | 4184 |
| BBa_K2570017 | Composite | dsrA+lacI+PlacI+hns+ProU+TyrB+LDH+rocG | The promoter induced by LacI will express global regulate protein HNS, which could repress ProU. To solve promoter leak problem, our membrane provided a salt-free environment to prevent the production of PLA. | 6964 |
| BBa_K2570018 | Composite | TyrB+LDH+rocG | Production of PLA. | 3599 |
In our project, we combined temperature promoter dsrABBa_K2570015 and salt-concentration promoter proUBBa_K2467001 as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA. dsrABBa_K2570015 is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher. [1]The salt-concentration promoter proUBBa_K2467001 provides sensitivity to the salt concentration to human skin. proU is a promoter existed in E.coli, induced by low salt concentration. And the H-NSBBa_K2570016 is a global-regulated protein which could bind to genome and specifically inhibit proU.[2]
This year the iGEM Edinburgh_OG team improved the BBa_K191006 part to fulfil the gold medal criteria.A core principle of the Biobrick foundation is to bring standardisation in a field that is constantly changing and evolving. Standardising biological parts and their assembly allows for projects to focus less on assembly of genetic constructs and more on design and testing.
[1] Temperature Sensing by the dsrA Promoter. JOURNAL OF BACTERIOLOGY, Nov. 2003, p. 6609–6614 DOI: 10.1128/JB.185.22.6609–6614.2003 [2] RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli. RNA Biology