Vector construction
![](http://highline.codal.kz/img/img-results.png)
For the vector construction, SQR gene was cloned into pSyn_6 vector, and through gel electrophoresis was checked the gene assembly. Figure 1 illustrates the bands of cloned pSyn_6 (5577 bp) in 1-4 wells between 3 and 4 ladder bands, which confirms the success of gene assembly. Also, cloned pSyn_6 plasmid was tested on a presence of SQR gene by PCR amplification using SQR primers. In Figure 2, we can see SQR bands (1271 bp) between 8 and 9 ladder bands that evidences of SQR presence in cloned pSyn_6 plasmid.
![](https://static.igem.org/mediawiki/2018/thumb/b/bb/T--NU_Kazakhstan--modifiedpSyn_6.png/747px-T--NU_Kazakhstan--modifiedpSyn_6.png)
Figure 1.Agarose gel electrophoresis (1%) of cloned pSyn_6 plasmid with SQR (5577 bp). (modifiedpsyn_6.png
![](https://static.igem.org/mediawiki/2018/2/26/T--NU_Kazakhstan--lss.png)