Team:HZAU-China/Collaborations
Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to use this system in their own projects. In the past two years, we have spent a great deal of time using it in our projects and accumulated a lot of experience (see more information in our previous wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in project on sharing the experience how we regulate the expression of CcaS and CcaR protein to control this optogenetic tool and what we achieved
We cooperated with the high school team, Worldshaper-Wuhan. We shared our projects and had several discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines. LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not. Secondly, we want to expire its applicability for which this biomarker can be widely utilized to detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS had speciality and universality. There were significant expression differences among 293T (the healthy cell), 769-P (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P and 786-O were higher than that of 293T, showing speciality and universality of IL7-AS. Through our team's suggestions and BALALA's experiment, their project improved and we also had a new biomarker and therapeutic target for future projects.
After the information we got from meetings and public investigation, we thought it’s necessary to enhance the targeting of engineered salmonella we used, we designed OmpA-RGD protein to achieve this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3 (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of Biochemistry and Cell Biology.
