Team:Aix-Marseille/Basic Part
We made three basic parts during this years iGEM competition coding for 2 different enzymes.
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BBa_K2718005
Methionine gamma-lyase (MGL) catalyzes the conversion of l-methionine into methanthiol, then the oxidation of methanethiol produces DMDS (Dimethyl disulfide) and DMTS ( Dimethyl trisulfide). BBa_K2718005 is an RFC 10 compatible part that we derived from BBa_K1493300 using PCR to add a his tag. To test activity we used the compositive part BBa_K2718006
BBa_K2718020
Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere to . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli. Part is RFC 25 standards. We test activiy with Schales' procedure [1].
BBa_K2718021
Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond to catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli, and there is his tag in C-ter. Part is RFC 25 standards. We test activiy with Schales' procedure[2].