Team:Edinburgh UG/Medal Criteria
Medal Criteria
Bronze Medal Criteria
#1 Registration and Giant Jamboree Attendance
We had a great iGEM season and see you all at the Giant Jamboree!
#2 Competition Deliverables
While reading our wiki, we hope you are excited to see our poster and presentation as well! We have also submitted a Judging form.
#3 Attributions
Our team is proud to state that we conceived the idea for Maxed OOT and subsequently designed and carried out all experiments ourselves. That said, there are many advisors who we would like to thank for helping to provide continuous advice and support, because, without them, we would not have been able to have achieved our goals. You can read more about people, teams and companies who helped us here.
#4 Characterization / Contribution
We successfully participated in InterLab study (see here). We performed and obtained successful results after performing both plate Reader and CFU and Flow Cytometry protocols.
Also, we characterised two parts from iGEM 2012 Paris Bettencourt. You can find out more about our improved parts here.
| Parts improved | Description | Supporting information |
|---|---|---|
| BBa_K914009 | P1003* Ser133->Amber Codon | contain a kanamycin resistance gene with one two serine -> amber codon mutation |
| BBa_K914018 | P1003** Kan resistant gene with 2 Amber Codon | contain a kanamycin resistance gene with two serine -> amber codon mutations |
Silver Medal Criteria
#1 Validated Part / Validated Contribution
Our validated parts:
| Parts Validated | Description | Supporting information |
|---|---|---|
| BBa_K2725016 | J23108 - SupD | Serine amber suppressor tRNA gene under J23108 Anderson promoter |
| BBa_K2725004 | FabV + Low expression cassette | Triclosan resistance gene from Vibrio fischeri |
| BBa_K2725013 | KanR 10* | P1003 kanamycin resistance with 10 amber stop codons |
#2 Collaboration
We have done Modelling Collaboration with team Vilnius-Lithuania. Besides attending lots of meetups, we also hosted a meetup with Newcastle iGEM 2018 team
#3 Human Practices
(See gold medal criteria #1 Integrated Human Practices)
Gold Medal Criteria
#1 Integrated Human Practices
The Edinburgh UG project looks to solve a fundamental problem facing synthetic biology - environmental release - with the development of a novel chassis. However, we also had to ensure our chassis had all the basic characteristics that a synthetic biologist would require. Therefore we consulted with a number of active researchers in synthetic biology in order to gauge what they would require from an ideal chassis. These consultations became a basis of our project design as a whole, and often changed the direction of each project part individually. We recorded these consultations and decisions on a timeline (link) so that anyone may follow and understand the progression of our project.
#2 Improve a Previous Part or Project
We improved the previous part BBa_K914009 which contain a kanamycin resistance gene with one serine -> amber codon mutations. By increasing the number of serine -> amber codon mutations to 5 in BBa_K2725012 they confer a decreased kanamycin resistance in the absence of a suppressor tRNA, reducing the leakiness of the gene.
| Parts | Description | Supporting information |
|---|---|---|
| BBa_K914009 | P1003* Ser133->Amber Codon | Improved the characterisation |
| BBa_K2725012 | P1003 Cassette with 5 amber stop codon | Improved the previous part BBa_K914009 |
#3 Model Your Project
Rational design of biobrick parts and protocols is an iGEM tenet that Team Edinburgh UG have embraced this year through mathematical modelling. The mechanistic modelling of our DNA degrading killswitch was used to check for viability and to decide which promoters and ribosome binding sites to use in the final parts. Modelling the probability of failure of our Semantic Containment system to prevent horizontal gene transfer allowed us to calculate the failure rate of our parts combining engineering safety techniques with Synthetic Biology. Using ordinal logistic regression we were able to use growth curve data to identify which of our Semantic Containment parts was present within an organism providing a quick way to diagnose instances of Semantic Containment failure. For more information please read here.
#4 Demonstration of Your Work
All aspects of our project combine to provide a novel chassis for Synthetic Biology: Maxicells. These maxicells are easy to use and can be safely deployed into the environment through the use of our Triple Lock System (link)
• We have assessed various methods of maxicell production and have concluded that they are easy to make through UV exposure and homing endonuclease action.
• The Triple Lock System - Colicin E2 kill switch, semantic containment and triclosan resistant plasmid backbone was produced
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