Team:Munich/chiinhibition1.html
In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/15| Participants: | Dominic Schwarz |
| Protocol: | Agarose gel, Restriction digest, Exonuclease V reaction | Notes: | Reaction was stoppedat at 10µl (50mM (2,82µl)) with EDTA (11mM), then heated on 70°C for 30 minutes. | Results: | Linear DNA was not degraded: we found out later that RecBCD was expired, but a positive control was missing. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/19| Participants: | Enikö Baligács |
| Protocol: | Agarose gel, Restriction digest, Exonuclease V reaction |
| Notes: | The experiment was repeated by Enikö Baligács and a positive control was added. |
| Results: | Linear DNA was not degraded: We found out that RecBCD was expired and not functional. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/07/02| Participants: | Julia Mayer |
| Protocol: | Agarose gel, Restriction digest, Exonuclease V reaction |
| Notes: | No notes |
| Results: | The Agarose gel was prepared wrong. Therefore, samples were lost and could not be evaluated. |
Linearizing pSB1C3_RFP
2018/08/01| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification |
| Notes: | EcoRI-HF |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers. Digested backbone was stored.
2018/08/02| Participants: | Enikö Baligács, Julia Mayer |
| Protocol: | Agarose gel, Restriction digest, Exonuclease V reaction |
| Notes: | The experiment was repeated with newly ordered recBCD |
| Results: | Chi6 oligomers inhibit RecBCD but are degraded within 30 minutes. We went on to show these results in cell extract. |
