Team:Munich/chibiobrick1.html
Forming Oligodimers from Single-Strand DNA chi6 Sequences
2018/06/01| Participants: | Dominic Schwarz |
| Protocol: | Oligodimerization |
| Notes: | Oligodimers were bought |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, Ligation, Chemical transformation, Miniprep, Sequencing |
| Notes: | pSB1C3 Backbone was generated by restriction with XbaI and SpeI. Chi6 Dimers were digested with XbaI & SpeI. Transformation of Dh5a |
| Results: | No sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | Digest with XbaI, SpeI Transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination (religation of backbound). |