Team:Edinburgh UG/Improve
Improve
BBa_K2725001
We improved the characterisation of a previous part: BBa_K771303, which contains the coding sequence of E. coli FabI. FabI is involved in type 2 fatty acid synthesis and has previously been characterised as a biosensor for triclosan, a common biocide. Overexpression of FabI has been shown to give resistance to triclosan, so we characterised the use of FabI as an alternative selection gene. Usage of triclosan instead of antibiotics is considerably cheaper, and safer for the environment.
BBa_K914009 & BBa_K914018
We improved the characterisation of the previous parts: BBa_K914009 &
We improved the previous parts: BBa_K914009 & BBa_K914018, which contain a kanamycin
resistance gene with one and two serine -> amber codon mutations, respectively. By
increasing the number of serine -> amber codon mutations to 5 in BBa_K2725012 and to 10
in BBa_K2725013, they confer a decreased kanamycin resistance in the absence of a
suppressor tRNA, reducing the leakiness of the gene. Feel free to leave us a comment on social media!BBa_K2725012 & BBa_K2725013
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