Team:UofGuelph/Contributions
Registry Contributions
2018 Registry Submissions
Sequencing of BBa_K747047
Background
iGEM Guelph's 2018 contribution to the registry was the sequence analysis of a BioBrick located in the 2018 Distribution Kit.
BioBrick K747047 was located in Plate 2, Well 12O. Our team chose to sequence this part as the Distribution Kit listed it as a Bad Sequence and for our team’s first characterization of a Registry Part, we thought that this part was a good candidate. Sequencing preparation was performed by our team, while the sequencing was performed at the Laboratory Services at the University of Guelph.
Methods
A PCR was set up according to the following recipe:
| Component | Volume (µL) |
|---|---|
| H2O | 20.4 |
| Phusion Buffer | 8.0 |
| dNTPs | 0.8 |
| VF2 | 0.2 |
| VR | 0.2 |
| BBa_DNA | 10 |
| Phusion | 0.4 |
| Total | 40 |
