Team:Munich/assembly3.html
T7 and 3S Phage Assembly in Self-Produced Cell-Extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie Schönberg von Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng 3S Phage: 330 ng Self-produced extract: E10 |
| Results: | T7 Phage: 1,6*10^8 PFU/mL 3S: did not work out. Decided to redo the experiment. |
T7 Phage Assembly in Self-Produced Cell-Extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie Schönberg von Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng, |
| Results: | T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL |
Reproduction of T7 Phage Assembly in E10 and P15
2018/10/03-2018/10/05| Participants: | Sophie Schönberg, Brigit Tunaj |
| Protocol: | Phage T7 Assembly TX/TL, Agar overlay plaque assay | Notes: | No notes. | Results: |
E 10: 2,1*10^8 PFU/mL P15:5,1*10^6 PFU/mL Lyophilized P15: 1*10^5 PFU/mL |
Reproduction of T7 Phage Assembly in E10 and P15
2018/10/07-2018/10/08| Participants: | Sophie Schönberg, Brigit Tunaj |
| Protocol: | Phage T7 Assembly TX/TL, Agar overlay plaque assay | Notes: | No notes. | Results: |
E 10 lyophilized: 1,4*10^6 PFU/mL P15:3,2*10^6 PFU/mL Lyophilized P15: 1,5*10^6 PFU/mL |
Reproduction of T7 Phage Assembly in E10 and P15
2018/10/07-2018/10/08| Participants: | Sophie Schönberg, Brigit Tunaj |
| Protocol: | Phage T7 Assembly TX/TL, Agar overlay plaque assay | Notes: | No notes. | Results: | E 10 lyophilized 2,7*10^6 PFU/mL. |
Assembly of various Phages in commercial cell extract
2018/10/10-2018/10/11| Participants: | Brigit Tunaj |
| Protocol: | Phage T7 Assembly TX/TL, Agar overlay plaque assay | Notes: | Phages: T5,T4,T7,3S, P2,P3 | Results: |
Titer: T5: 6*10^7 PFU/mL T7: 1,8*10^8 PFU/mL T4: 4*10^9 PFU/mL 3S:5*^06 PFU/mL P3:3*10^7 PFU/mL P2:2,1*10^9 PFU/mL |
Engineered MS2 Phage in self produced cell extract P15
2018/10/10| Participants: | Brigit Tunaj |
| Protocol: | Phage T7 Assembly TX/TL, Agar overlay plaque assay | Notes: | No notes. | Results: | 3*10^7 PFU/mL |
TEM picture of various phages
2018/10/10 -2018/10/17| Participants: | Brigit Tunaj, with supervisor Kilian Vogele |
| Protocol: | TEM preparation | Notes: | No notes. | Results: | Pictures are shown in results part |
TEM picture of various phages
2018/10/10 -2018/10/17| Participants: | Brigit Tunaj, with supervisor Kilian Vogele |
| Protocol: | TEM preparation | Notes: | No notes. | Results: | Pictures are shown in results part |