Team:BrockU

Welcome to the BrockU iGEM Page!

About Us

The Brock iGEM Team is a group of passionate undergraduate students who are looking to experience the world of research. This interest has brought together a wide variety of disciplines that have formed a coherent team based off our shared interests in various biotechnological and molecular biological techniques to enhance our studies during our undergraduate degrees. We are currently working towards preparing a project to bring to display at the iGEM conference in 2018, that will allow us to improve ourselves for our varying interests.

Project Summary

Flip recombinase (Flippase) is an enzyme that is capable of inducing site-specific recombination. When expressed, Flippase has the ability to excise a sequence of DNA that lies between two specific recombinase cleavage sites, termed FRT sites. Flippase induces excision of the entire intervening sequence of DNA between these sites. The overall goal of our project is to engineer a light-activatable Flippase by splitting the enzyme in two portions that each carry a genetically-encoded Optogenetic tag. These Optogenetic tags will allow for light-dependent induction of the association of the two Flip recombinase fragments, resulting in the reconstitution of Flip functionality. This construct, termed Opto-Flip, will allow us to control the function of Flip-mediated recombination precisely in space and time. Successful engineering of Opto-Flip will provide a light-gated system that  can serve as a useful tool for a broad set of applications in cell biology and molecular genetics.

Before you start

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Uploading pictures and files

You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
  • Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples: