Team:Unesp Brazil
iGEM Unesp Brazil
Hope is a robust framework to engineer living therapeutics to treat metabolic disorders. Going from design, fine-tuned expression, biocontainment to real-world application, Hope offers an easy, modular, robust and open-source solution to engineer probiotics and validate them, bringing the next generation of therapeutics one step closer.
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Sense and control
Fine-tuned and orthogonal regulatory circuit engineered to compute a specific input and control the expression of the gene of interest. The device is modular, making it easy to reengineer for different applications.
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Production
Standardized device to drive production and delivery of therapeutic proteins in the gut. A signal peptide addresses the therapeutic protein to the gut environment, and a cell penetrating peptide drives it through the intestinal epithelium.
read more >
Biocontainment
Once the microorganism is released from human body and exposed to light, a light-inducible system based on the CRISPR/Cas9 machinery will be activated to kill the cell and destroy its synthetic DNA.
read more >
Gut simulation
To test and validate the functionality of genetically engineered probiotics, we designed and built a do-it-yourself and low-cost bioreactor that simulates the human gut and the microbiome.
read more >
As a proof of concept for our framework, we use Hope to build an alternative treatment for type 1 diabetes. Therefore, we genetically engineered Bacillus subtilis to activate gene expression upon glucose/fructose induction, and produce and secrete a single-chain insulin analog that can be absorbed by intestinal cells. Come with us to learn all about our work!
Hope is a robust framework to engineer living therapeutics to treat metabolic disorders. Going from design, fine-tuned expression, biocontainment to real-world application, Hope offers an easy, modular, robust and open-source solution to engineer probiotics and validate them, bringing the next generation of therapeutics one step closer.
read more >
Sense and control
Fine-tuned and orthogonal regulatory circuit engineered to compute a specific input and control the expression of the gene of interest. The device is modular, making it easy to reengineer for different applications.
read more >
Production
Standardized device to drive production and delivery of therapeutic proteins in the gut. A signal peptide addresses the therapeutic protein to the gut environment, and a cell penetrating peptide drives it through the intestinal epithelium.
read more >
Biocontainment
Once the microorganism is released from human body and exposed to light, a light-inducible system based on the CRISPR/Cas9 machinery will be activated to kill the cell and destroy its synthetic DNA.
read more >
Gut simulation
To test and validate the functionality of genetically engineered probiotics, we designed and built a do-it-yourself and low-cost bioreactor that simulates the human gut and the microbiome.
read more >
As a proof of concept for our framework, we use Hope to build an alternative treatment for type 1 diabetes. Therefore, we genetically engineered Bacillus subtilis to activate gene expression upon glucose/fructose induction, and produce and secrete a single-chain insulin analog that can be absorbed by intestinal cells. Come with us to learn all about our work!