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InterLab

Fifth International InterLab Measurement Study

Aim

This year, we participated in the Fifth international InterLab Measurement Study. This collective study among the world aims at promoting synthetic biology into a real engineering discipline by improving the repeatability and reliability of current measurement tools. Green Fluorescent Protein (GFP) signal is harnessed as a measurement marker in most synthetic biology study. Be that as it may, variability and abstract ways of presentation make it harder to repeat and duplicate the results. By collecting data generated by the same protocol and standardised equipment and settings, iGEM community could utilise it to reduce lab-to-lab variability of GFP measurement. This year, the aim of InterLab study is to normalising fluorescence measurements using absolute cell count and colony-forming units (CFU) instead of OD. One of the challenges in this InterLab study is to ensure the correct value of absorbance and OD. In order to standardise the signals and the data, an initial OD and hence a correct cell concentration are crucial.

Graph 1. Standard curve of GFP signals produced by fluorescein to standardise relate cell concentration and GFP signals

Graph 2. Absorbance changes of different samples over 6 hours

Graph 3. Fluorescence signal changes of different samples over 6 hours