Team:Hong Kong HKU/Part Collection
Part Collection
Nano Drug Carrier-AS (NDC-AS)
Bacterial synthesis of DNA aptamer AS1411
This BioBrick encodes Strand 2-AS, which is a part of the nanostructure tetrahedral base, with nucleolin aptamer AS1411 attached to its 5' end.
This part is one of the few iGEM BioBricks designed for synthesis of DNA aptamers inside E. coli. Over the years, many iGEM teams have registered BioBricks containing only the aptamer sequences they designed. The Strand 2-AS sequence in this part can be easily substituted with any other DNA aptamer sequences using the assembly standards desribed above.
This BioBrick encodes Strand 1-AS, which is a modified NDC Strand 1 with extended binding sequence to Strand 5-AS to increase strand displacement specificity.
This BioBrick encodes Strand 5-AS, which is modified from NDC Strand 5 with extended binding sequence to Strand 1-AS of the tetrahedral body. Part of this strand is a displacement toehold complimentary to miR21. The 5' section next to the toehold is complimentary to another target, miR217, while the 3' end binds complimentarily to part of Strand 1.
BioBrick coding NDC/NDC-AS strand 3, part of the tetrahedral body of the nanostructure.
BioBrick coding NDC/NDC-AS strand 4, part of the tetrahedral body of the nanostructure.
ETHERNO
BBa_K2592005 BBa_K2592006 BBa_K2592002 BBa_K2592003 BBa_K2592007
Nano Drug Carrier (NDC)
BioBrick coding NDC Strand 1, which holds Strand 5 in place on the tetrahedral nanostructure by complimentary base pairing.
BioBrick coding NDC strand 2, part of the tetrahedral body of the nanostrcuture.
BioBrick coding NDC/NDC-AS strand 3, part of the tetrahedral body of the nanostructure.
BioBrick coding NDC/NDC-AS strand 4, part of the tetrahedral body of the nanostructure.
BioBrick coding NDC strand 5, part of which is a displacement toehold complimentary to miR21. The 5' section next to the toehold is complimentary to another target, miR217. The 3' end binds complimentarily to part of Strand 1.