After developing our idea for electronic control of gene expression we found a previous method was developed by Tschirhart et al. (2017). Although several barriers prevent this systems general use in synthetic biology. It only functions in anaerobic conditions, did not demonstrate spatial control, used an expensive electrochemical set-up, was not assembled modularly and provided no part library to modify the biological response. Throughout our wet lab experiments we aimed to overcome these problems to generate a modular, affordable and aerobic electrogenetic control system capable of patterning cells capable of achieving spatial patterning.
General Circuit Design