This is a collection of all parts we worked with, for more information about the parts, their function and results, please visit the results page or the parts homepage on parts.igem.org.
- Composite parts: BBa_K2671420
- Basic Parts: BBa_K2671337
- Improved parts: BBa_K2671000
- Verified collaboration parts: BBa_K2602021, BBa_K2602024, BBa_K2602026
- Verified biobrick: BBa_I746909, BBa_K173007
Primers used for all our experiments can be found below.
- Vector reverse (VR): attaccgcctttgagtgagc (used for sequencing and colony screening)
- Vector forward 2 (VF2): tgccacctgacgtctaagaa (used for sequencing and colony screening)
- Backbone SNP reverse (BSNPR): ctctagaagcggccgcgaat (used to amplify pSB4A5 from the distributed plates)
- Backbone ENX forward (BENXF): tactagtagcggccgctg (used to amplify pSB4A5 from the distributed plates)
- Liu iGEM17 site directed mutagenesis reverse (SDMR): cccgaaccgctttacttg (used for the improvement)
- Liu iGEM17 site directed mutagenesis forward (SDMR): ctcaggaagcactagtggctcggac (used for the improvement)
- Liu iGEM17 mNG forward (mNG-F): ggactggtgcaggtcgaaga (used for colony screening of the improvement)